用于检测人抗伤寒沙门氏菌Vi抗原抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for detection of human antibodies to Salmonella typhi Vi antigen.
作者信息
Barrett T J, Blake P A, Brown S L, Hoffman K, Llort J M, Feeley J C
出版信息
J Clin Microbiol. 1983 Apr;17(4):625-7. doi: 10.1128/jcm.17.4.625-627.1983.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to Salmonella typhi Vi antigen in human serum, and the results were compared with those from a previously described hemagglutination assay (HA). The ELISA detected Vi antibodies at a titer of greater than or equal to 20 in 40 (52%) of 77 sera from typhoid fever patients, whereas the HA gave titers of greater than or equal to 20 in 35 (47%). Determination of titers of serum specimens from 170 persons without typhoid fever revealed Vi antibody titers of greater than or equal to 20 in 4 (2.3%) by the ELISA and 3 (1.7%) by the HA. Unlike the sensitized erythrocytes used in the HA, the ELISA reagents have a shelf life of greater than or equal to 1 year. The ELISA may be preferred by some laboratories, especially those already performing other ELISA tests.
摘要
开发了一种酶联免疫吸附测定法（ELISA）来检测人血清中针对伤寒杆菌Vi抗原的抗体，并将结果与先前描述的血凝试验（HA）的结果进行比较。ELISA在77份伤寒热患者血清中的40份（52%）中检测到效价大于或等于20的Vi抗体，而HA在35份（47%）中检测到效价大于或等于20的Vi抗体。对170名无伤寒热患者的血清标本进行效价测定，ELISA检测到4份（2.3%）效价大于或等于20的Vi抗体，HA检测到3份（1.7%）。与HA中使用的致敏红细胞不同，ELISA试剂的保质期大于或等于1年。一些实验室可能更喜欢使用ELISA，特别是那些已经在进行其他ELISA检测的实验室。
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