Moroff G, Meryman H T
Vox Sang. 1979;36(4):244-51. doi: 10.1111/j.1423-0410.1979.tb04432.x.
Glycerolization of freshly collected human erythrocytes leads to a reduction in ATP levels which return to their original values following deglycerolization. The reduction in ATP levels is largely prevented by pyruvate but not by inosine and glucose, singly or in combination. This implies that the ATP lesion associated with glycerolization reflects mainly a decrease in the NAD/NADH ratio and accordingly a reduced glyceraldehyde-3-phosphate dehydrogenase activity. 2,3-DPG levels were not influenced by glycerolization and deglycerolization. Erythrocytes depleted of ATP in the presence of glycerol can have their ATP levels repleted by either pyruvate or inosine, but require the presence of both compounds for maintenance during a post-rejuvenation incubation period. This indicates that the prolonged presence of glycerol influences both the NAD/NADH ratio and the carbon-containing intermediates of glycolysis. Glycerol did not influence ATP repletion of erythrocytes stored at 4 degrees C for 20 days by inosine in combination with pyruvate, but substantially decreased the repletion of their 2,3-DPG levels.
新鲜采集的人红细胞进行甘油化处理会导致ATP水平降低，脱甘油处理后ATP水平会恢复到原始值。丙酮酸可在很大程度上防止ATP水平降低，但肌苷和葡萄糖单独或联合使用均无此作用。这表明与甘油化相关的ATP损伤主要反映了NAD/NADH比值的降低，进而导致甘油醛-3-磷酸脱氢酶活性降低。2,3-DPG水平不受甘油化和脱甘油处理的影响。在甘油存在的情况下ATP耗尽的红细胞，其ATP水平可通过丙酮酸或肌苷补充，但在恢复后孵育期需要同时存在这两种化合物才能维持。这表明甘油的长期存在会影响NAD/NADH比值和糖酵解的含碳中间产物。甘油不影响肌苷与丙酮酸联合对在4℃储存20天的红细胞的ATP补充，但会显著降低其2,3-DPG水平的补充。
