福氏志贺菌2a中的突变导致电泳迁移率增加。
Mutation in Shigella flexneri 2a resulting in increased electrophoretic mobility.
作者信息
Corwin L M, Talevi L A
出版信息
Infect Immun. 1972 May;5(5):798-802. doi: 10.1128/iai.5.5.798-802.1972.
Abstract
An avirulent mutant of Shigella flexneri 2a, when grown in broth containing calcium ions, was found to have a three- to fourfold increase in electrophoretic mobility toward the anode when compared to its virulent parent. Under these same growth conditions, it was found that the avirulent strain was made more electronegative in the presence of ethylenediaminetetraacetic acid (EDTA). EDTA had no effect on the electrophoretic mobility of the virulent strain. In the absence of added calcium in the growth medium, no differences between the strains were observed. The alteration which has resulted in an increased negative charge has also stabilized the avirulent strain against loss of lipopolysaccharide from EDTA treatment.
摘要
当弗氏志贺菌2a的无毒突变株在含有钙离子的肉汤中生长时，发现与有毒亲代相比，其向阳极的电泳迁移率增加了三到四倍。在相同的生长条件下，发现无毒菌株在乙二胺四乙酸（EDTA）存在下带更多负电荷。EDTA对有毒菌株的电泳迁移率没有影响。在生长培养基中不添加钙的情况下，未观察到菌株之间的差异。导致负电荷增加的这种变化也使无毒菌株在经EDTA处理后更稳定，不易丢失脂多糖。
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2
3
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4
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