Bohin J P, Rigomier D, Schaeffer P
J Bacteriol. 1976 Aug;127(2):934-40. doi: 10.1128/jb.127.2.934-940.1976.
The growth rate of Bacillus subtilis is lowered but the final cell yield is unchanged when certain concentrations of ethanol are present in the culture medium. At the concentration allowing growth at half-maximal rate, practically no spores are formed. Blockage of spore formation generally occurs at stage 0-I. Sensitivity to ethanol of the capacity to form spores is limited, in a nonsynchronized culture, to a period of at most 45 min around t1. Postexponential events such as excretion of certain enzymes and modification of ribonucleic acid polymerase are altered or suppressed in the presence of ethanol, possibly as the results of a physical change upon the cell membrane. In effect, ethanol is turning wild-type cells into phenocopies of spoO mutants.
当培养基中存在一定浓度的乙醇时，枯草芽孢杆菌的生长速率降低，但最终细胞产量不变。在允许以最大生长速率一半的速度生长的浓度下，几乎不形成孢子。孢子形成的阻断通常发生在0-I阶段。在非同步培养中，形成孢子能力对乙醇的敏感性仅限于t1前后最多45分钟的时间段。在乙醇存在的情况下，诸如某些酶的分泌和核糖核酸聚合酶的修饰等指数后期事件会发生改变或受到抑制，这可能是细胞膜发生物理变化的结果。实际上，乙醇正在将野生型细胞转变为spoO突变体的拟表型。
