6-（对羟基苯偶氮）尿嘧啶：噬菌体感染的枯草芽孢杆菌中宿主DNA复制的选择性抑制剂。
6-(p-hydroxyphenylazo)-uracil: a selective inhibitor of host DNA replication in phage-infected Bacillus subtilis.
作者信息
Brown N C
出版信息
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1454-61. doi: 10.1073/pnas.67.3.1454.
Abstract
The azopyrimidine, 6-(p-hydroxyphenylazo)-uracil, inhibits the replication of bacterial DNA selectively, completely, and reversibly, and has no apparent effects on the metabolism of other cellular macromolecules thus far examined. The mechanism of its action has been investigated in uninfected and phage-infected Bacillus subtilis, and the compound appears to be specific for a host function. In cells infected with virulent phage the synthesis of phage DNA proceeds normally, while residual host DNA synthesis is completely blocked. The drug-sensitive host site retains its sensitivity even after partial disruption of the cell by lysozyme treatment.
摘要
偶氮嘧啶6-(对羟基苯偶氮)-尿嘧啶能选择性、完全且可逆地抑制细菌DNA的复制，并且迄今为止对所检测的其他细胞大分子的代谢没有明显影响。已在未感染和噬菌体感染的枯草芽孢杆菌中研究了其作用机制，该化合物似乎对宿主功能具有特异性。在感染烈性噬菌体的细胞中，噬菌体DNA的合成正常进行，而残留的宿主DNA合成则完全被阻断。即使在用溶菌酶处理使细胞部分裂解后，药物敏感的宿主位点仍保持其敏感性。
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