Yang C Y
Appl Microbiol. 1972 Dec;24(6):885-90. doi: 10.1128/am.24.6.885-890.1972.
Cultures of Aspergillus flavus and aflatoxins were destroyed by a commercial bleach (Clorox; active ingredient, NaOCl) or analytical reagent grade NaOCl at 7.0 x 10(-3) M NaOCl in 5 days. Addition of Clorox or NaOCl at 2.8 x 10(-3) M to the fungal growth medium prior to inoculation completely inhibited the fungal growth. Aflatoxin production was inversely proportional to the logarithm of NaOCl concentration and time of treatment. Clorox and NaOCl were equally effective on aflatoxins, but fungal cells were lysed more readily by Clorox than by NaOCl. Mycelia older than 8 days lysed more readily than younger ones. Most conidia survived concentrations below 1.4 x 10(-3) M. The lowest effective concentration for a 2-hr treatment was 8.8 x 10(-3) M which is well below the Clorox concentration recommended for routine laboratory decontamination of aflatoxins. Mice and rats injected with aflatoxins and aflatoxins incompletely destroyed by Clorox died within 72 hr and had typical liver and kidney damage caused by aflatoxins. However, animals injected with NaOCl or Clorox or Clorox-destroyed aflatoxin extracts survived and showed no obvious liver or kidney damage.
黄曲霉培养物和黄曲霉毒素在5天内被市售漂白剂（高乐氏；有效成分，次氯酸钠）或分析纯级次氯酸钠（浓度为7.0×10⁻³ M）破坏。在接种前向真菌生长培养基中添加2.8×10⁻³ M的高乐氏或次氯酸钠可完全抑制真菌生长。黄曲霉毒素的产生与次氯酸钠浓度的对数和处理时间成反比。高乐氏和次氯酸钠对黄曲霉毒素的效果相同，但高乐氏比次氯酸钠更容易使真菌细胞裂解。8天以上的菌丝体比年轻的菌丝体更容易裂解。大多数分生孢子在浓度低于1.4×10⁻³ M时存活。2小时处理的最低有效浓度为8.8×10⁻³ M，这远低于推荐用于黄曲霉毒素常规实验室去污的高乐氏浓度。注射了黄曲霉毒素以及未被高乐氏完全破坏的黄曲霉毒素的小鼠和大鼠在72小时内死亡，并有黄曲霉毒素引起的典型肝肾损伤。然而，注射了次氯酸钠、高乐氏或经高乐氏破坏的黄曲霉毒素提取物的动物存活下来，且未表现出明显的肝肾损伤。
