Strauss R R, Paul B B, Jacobs A A, Sbarra A J
Infect Immun. 1972 Jan;5(1):120-6. doi: 10.1128/iai.5.1.120-126.1972.
Spleen cell suspensions from AKR and CD-1 mice contain peroxidase activity as determined by guaiacol oxidation. This activity is found predominately in the 20,000 x g pellet fraction of spleen cell homogenates. In the presence of H(2)O(2) and chloride ion at acidic pH, splenic peroxidase mediates the oxidation of d- or l-alanine to CO(2), NH(3), and acetaldehyde. The same reaction mixture without added amino acid can kill both gram-positive and gram-negative bacteria. The conditions for both reactions are similar. Both have an absolute requirement for H(2)O(2) and chloride ion, neither is active at neutral or alkaline pH, and both are inhibited by the sulfonic amino acid taurine. In these aspects, splenic peroxidase is qualitatively similar in its activity to myeloperoxidase (MPO) from polymorphonuclear leukocytes. It is quantitatively different from MPO in that the latter is more potent on a per guaiacol unit basis with respect to both amino acid oxidation and bactericidal activity. Still another quantitative difference is that splenic peroxidase requires 0.1 m NaCl for activity, whereas MPO functions with as little as 0.005 m NaCl. Splenic peroxidase and MPO both appear to differ qualitatively from horseradish peroxidase in that the latter enzyme does not mediate amino acid oxidation.
通过愈创木酚氧化法测定发现，AKR小鼠和CD-1小鼠的脾细胞悬液含有过氧化物酶活性。这种活性主要存在于脾细胞匀浆经20,000×g离心后的沉淀部分。在酸性pH条件下，脾过氧化物酶在过氧化氢（H₂O₂）和氯离子存在时，可介导d-或l-丙氨酸氧化为二氧化碳（CO₂）、氨（NH₃）和乙醛。相同的反应混合物在不添加氨基酸时，可杀死革兰氏阳性菌和革兰氏阴性菌。两种反应的条件相似。二者都绝对需要H₂O₂和氯离子，在中性或碱性pH条件下均无活性，且都受到磺酸氨基酸牛磺酸的抑制。在这些方面，脾过氧化物酶的活性在性质上与多形核白细胞的髓过氧化物酶（MPO）相似。二者在数量上有所不同，就愈创木酚单位而言，MPO在氨基酸氧化和杀菌活性方面更强。另一个数量上的差异是，脾过氧化物酶活性需要0.1 m NaCl，而MPO在低至0.005 m NaCl的条件下就能发挥作用。脾过氧化物酶和MPO在性质上似乎都与辣根过氧化物酶不同，因为后者不能介导氨基酸氧化。
