Cheley S, Anderson R, Cupples M J, Chan E C, Morris V L
Virology. 1981 Jul 30;112(2):596-604. doi: 10.1016/0042-6822(81)90305-6.
A major polyadenylated viral RNA of approximately 0.8 × 10 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct translation in a cell-free, reticulocyte-derived system. Single stranded P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.
通过在甲酰胺中进行制备性聚丙烯酰胺凝胶电泳，从感染鼠肝炎病毒（A59）的细胞中分离出一种约0.8×10道尔顿的主要多聚腺苷酸化病毒RNA。在无细胞的、源自网织红细胞的系统中进行直接翻译，结果表明这种RNA编码病毒核衣壳蛋白。从这种RNA制备了单链P标记的互补DNA，并证明其具有病毒特异性。在Northern印迹实验中使用这种互补DNA，我们能够鉴定出六种主要的病毒特异性细胞内RNA种类，其估计分子量分别为0.8、1.1、1.4、1.6、3和4×10道尔顿。所有这些RNA种类都是多聚腺苷酸化的。我们的结果支持这样一种观点，即冠状病毒感染的细胞含有多个共享共同序列的细胞内多聚腺苷酸化RNA。
