Intracellular murine hepatitis virus-specific RNAs contain common sequences.
作者信息
Cheley S, Anderson R, Cupples M J, Chan E C, Morris V L
出版信息
Virology. 1981 Jul 30;112(2):596-604. doi: 10.1016/0042-6822(81)90305-6.
Abstract
A major polyadenylated viral RNA of approximately 0.8 × 10 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct translation in a cell-free, reticulocyte-derived system. Single stranded P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.
摘要
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