溶液中超螺旋脱氧核糖核酸的三维结构。从散射光的角度分布获得的证据。
The three-dimensional structure of supercoiled deoxyribonucleic acid in solution. Evidence obtained from the angular distribution of scattered light.
作者信息
Jolly D J, Campbell A M
出版信息
Biochem J. 1972 Jul;128(3):569-78. doi: 10.1042/bj1280569.
Abstract
- The size and shape of superhelical double-stranded circular DNA from bacteriophage ØX174 were investigated by light-scattering. The molecular weight of the DNA is 3.17x10(6) and the root-mean-square radius is 103.5nm. 2. The light-scattering envelopes of various theoretical three-dimensional models for such DNA molecules were calculated by repetitive computational techniques, and the results were compared with the experimental findings. 3. It is concluded that the structure of supercoiled DNA containing -12 superhelical turns in buffer of I0.2 corresponds best to one of the more compact models for superhelix structure such as the branched model, and the commonly employed straight interwound superhelix model is incompatible with the experimental results, at the superhelix density found.
摘要
- 通过光散射研究了噬菌体ØX174超螺旋双链环状DNA的大小和形状。该DNA的分子量为3.17×10⁶，均方根半径为103.5nm。2. 采用重复计算技术计算了此类DNA分子各种理论三维模型的光散射包络，并将结果与实验结果进行了比较。3. 得出结论：在I0.2缓冲液中含有-12个超螺旋圈的超螺旋DNA结构最符合超螺旋结构的更紧密模型之一，如分支模型，而常用的直链缠绕超螺旋模型与所发现的超螺旋密度下的实验结果不相符。
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