脱氧核糖核酸柔韧性的光散射研究。一种小型环状脱氧核糖核酸分子的溶液性质。
Light-scattering studies on deoxyribonucleic acid flexibility. The solution properties of a small circular deoxyribonucleic acid molecule.
作者信息
Jolly D J, Campbell A M
出版信息
Biochem J. 1972 Dec;130(4):1019-28. doi: 10.1042/bj1301019.
Abstract
Previous investigations on the persistence length of DNA in solution have revealed large discrepancies between hydrodynamic results and those from light-scattering techniques which have potentially a greater resolving power. The information obtained from experiments on a small circular DNA molecule has resolved these discrepancies. The non-superhelical circular double-stranded DNA molecule from bacteriophage [unk]X174-infected cells is small enough to permit accurate light-scattering extrapolations, and its solutions have negligible anisotropy. The persistence length obtained from experimental investigations on this molecule is comparable with that obtained by hydrodynamic techniques, even with variation of the excluded-volume factor.
摘要
先前对溶液中DNA持久长度的研究表明，流体动力学结果与光散射技术结果之间存在很大差异，而光散射技术可能具有更高的分辨能力。从小型环状DNA分子实验中获得的信息解决了这些差异。来自噬菌体[unk]X174感染细胞的非超螺旋环状双链DNA分子足够小，便于进行精确的光散射外推，并且其溶液的各向异性可忽略不计。即使排除体积因子有所变化，通过对该分子进行实验研究获得的持久长度与流体动力学技术获得的持久长度相当。
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本文引用的文献
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Application of light scattering to biological systems: deoxyribonucleic acid and the muscle proteins.光散射在生物系统中的应用：脱氧核糖核酸与肌肉蛋白
Adv Biol Med Phys. 1958;6:431-551. doi: 10.1016/b978-1-4832-3112-9.50014-1.
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Conformation studies on the sodium and cesium salts of calf thymus deoxyribonucleic acid (DNA).小牛胸腺脱氧核糖核酸（DNA）钠盐和铯盐的构象研究。
Biopolymers. 1966 Apr-May;4(4):429-40. doi: 10.1002/bip.1966.360040404.
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J Mol Biol. 1968 Jul 14;35(1):111-29. doi: 10.1016/s0022-2836(68)80041-5.
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