Westerberg S C, Smith C B, Wiley B B, Jensen C
Infect Immun. 1972 Jun;5(6):840-6. doi: 10.1128/iai.5.6.840-846.1972.
The effects produced by single and mixed infections with Mycoplasma pulmonis and influenza A/PR-8 virus were studied in mouse tracheal organ cultures. M. pulmonis multiplied in the tracheal organ cultures, producing inhibition of ciliary activity and histologic tissue damage. The organism grew in close association with the cell membranes but did not appear to attach directly to the membranes or the cilia. Influenza A virus also replicated in tracheal organ cultures, producing ciliary inhibition and more extensive cytopathologic changes. Virus particles were seen by electron microscopy to attach to and cause clumping of the cilia. Simultaneous infection of the organ cultures with mycoplasma and virus resulted in more rapid inactivation of ciliary activity and greater tissue damage than occurred when the cultures were infected with only mycoplasma or virus. Presence of the virus appeared to have no effect on the growth of the mycoplasma; however, the mycoplasma partially interfered with virus replication.
在小鼠气管器官培养物中研究了肺炎支原体和甲型流感病毒A/PR - 8单独感染及混合感染所产生的影响。肺炎支原体在气管器官培养物中繁殖，导致纤毛活动受到抑制和组织学损伤。该微生物与细胞膜紧密相连生长，但似乎并未直接附着于细胞膜或纤毛。甲型流感病毒也在气管器官培养物中复制，导致纤毛抑制和更广泛的细胞病变变化。通过电子显微镜观察到病毒颗粒附着并导致纤毛聚集。与仅感染支原体或病毒相比，器官培养物同时感染支原体和病毒会导致纤毛活动更快失活和更严重的组织损伤。病毒的存在似乎对支原体的生长没有影响；然而，支原体部分干扰了病毒复制。
