The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.
作者信息
Brocklehurst K, Crook E M, Kierstan M
出版信息
Biochem J. 1972 Jul;128(4):979-82. doi: 10.1042/bj1280979.
Abstract
摘要
相似文献
1
The mutability of stem bromelain: evidence for perturbation by structural transitions of the parameters that characterize the reaction of the essential thiol group of bromelain with 2,2'-dipyridyl disulphide.菠萝蛋白酶原的可变性：菠萝蛋白酶中必需巯基与2,2'-二吡啶二硫化物反应特征参数的结构转变引起扰动的证据。
Biochem J. 1972 Jul;128(4):979-82. doi: 10.1042/bj1280979.
2
The thiol group of bovine serum albumin. High reactivity at acidic pH as measured by the reaction with 2,2'-dipyridyl disulphide.牛血清白蛋白的巯基。通过与2,2'-二吡啶二硫化物反应测定，在酸性pH下具有高反应活性。
Biochim Biophys Acta. 1975 Aug 19;400(2):433-8. doi: 10.1016/0005-2795(75)90199-3.
3
Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.木瓜蛋白酶和L-半胱氨酸与2,2'-二吡啶二硫化物和4,4'-二吡啶二硫化物反应中各种质子态的反应活性：木瓜蛋白酶中半胱氨酸-25残基的未电离硫醇基团因其与组氨酸-159-天冬酰胺-175氢键系统相互作用而产生亲核反应活性的证据。
Biochem J. 1972 Jun;128(2):471-4. doi: 10.1042/bj1280471.
4
Chemical modification of stem bromelain I-1 and fruit bromelain A with 2-hydroxy-5-nitrobenzyl bromide, tetranitromethane, and hydrogen peroxide.
J Biochem. 1975 Sep;78(3):627-35. doi: 10.1093/oxfordjournals.jbchem.a130949.
5
Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe.2,2'-二吡啶二硫化物的中性和阳离子形式对硫醇盐阴离子的反应活性。通过三质子态反应探针检测猕猴桃蛋白酶、木瓜蛋白酶和无花果蛋白酶活性中心之间的差异。
Biochem J. 1979 Nov 1;183(2):233-8. doi: 10.1042/bj1830233.
6
Preparation of cathepsins B and H by covalent chromatography and characterization of their catalytic sites by reaction with a thiol-specific two-protonic-state reactivity probe. Kinetic study of cathepsins B and H extending into alkaline media and a rapid spectroscopic titration of cathepsin H at pH 3-4.通过共价色谱法制备组织蛋白酶B和H，并通过与硫醇特异性双质子态反应性探针反应来表征其催化位点。对组织蛋白酶B和H在碱性介质中的动力学研究以及在pH 3-4条件下对组织蛋白酶H的快速光谱滴定。
Biochem J. 1985 Apr 15;227(2):511-9. doi: 10.1042/bj2270511.
7
Reactivity of the thiol group in human and bovine albumin at pH 3--9, as measured by exchange with 2,2'-dithiodipyridine.通过与2,2'-二硫代二吡啶交换来测定人血清白蛋白和牛血清白蛋白中硫醇基团在pH 3至9条件下的反应活性。
Eur J Biochem. 1980 May;106(1):291-5. doi: 10.1111/j.1432-1033.1980.tb06022.x.
8
Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.木瓜凝乳蛋白酶A。通过共价色谱法进行纯化及研究，并利用双质子态反应探针动力学、稳态动力学以及某些二硫代酰基衍生物的共振拉曼光谱进行表征。
Biochem J. 1986 Jan 1;233(1):119-29. doi: 10.1042/bj2330119.
9
Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.以2,2'-二吡啶二硫化物和4-氯-7-硝基苯并呋喃为反应探针，表征番木瓜蛋白酶A为番木瓜（Carica papaya L.）的一种半胱氨酸蛋白酶，其活性中心特性与木瓜蛋白酶不同。使用双质子态亲电试剂鉴定催化位点的巯基。
Biochem J. 1982 Jul 1;205(1):205-11. doi: 10.1042/bj2050205.
10
Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols.木瓜蛋白酶及低分子量硫醇与某些芳香族二硫化物的反应。2,2'-二吡啶基二硫化物作为木瓜蛋白酶便捷的活性位点滴定剂，即使在存在其他硫醇的情况下也适用。
Biochem J. 1973 May;133(1):67-80. doi: 10.1042/bj1330067.
引用本文的文献
1
Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.以2,2'-二吡啶二硫化物和4-氯-7-硝基苯并呋喃为反应探针，表征番木瓜蛋白酶A为番木瓜（Carica papaya L.）的一种半胱氨酸蛋白酶，其活性中心特性与木瓜蛋白酶不同。使用双质子态亲电试剂鉴定催化位点的巯基。
Biochem J. 1982 Jul 1;205(1):205-11. doi: 10.1042/bj2050205.
2
Covalent chromatography. Preparation of fully active papain from dried papaya latex.共价色谱法。从干燥的木瓜乳胶中制备全活性木瓜蛋白酶。
Biochem J. 1973 Jul;133(3):573-84. doi: 10.1042/bj1330573.
3
Reactions of L-ergothioneine and some other aminothiones with2,2'-and 4,4'-dipyridyl disulphides and of L-ergothioneine with iodoacetamide. 2-Mercaptoimidazoles, 2- and 4-thiopyridones, thiourea and thioacetamide as highly reactive neutral sulphur nucleophils.L-麦角硫因及其他一些氨基硫酮与2,2'-和4,4'-联吡啶二硫化物的反应以及L-麦角硫因与碘乙酰胺的反应。2-巯基咪唑、2-和4-硫代吡啶酮、硫脲和硫代乙酰胺作为高反应性中性硫亲核试剂。
Biochem J. 1974 Apr;139(1):221-35. doi: 10.1042/bj1390221.
4
The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester.茎菠萝蛋白酶的结构与机制。纯化茎菠萝蛋白酶的均一性评估、分子量测定以及菠萝蛋白酶催化N-苄氧羰基-L-苯丙氨酰-L-丝氨酸甲酯水解的动力学分析。
Biochem J. 1974 Dec;143(3):575-86. doi: 10.1042/bj1430575.
5
Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses.菠萝蛋白酶和木瓜蛋白酶催化N-苯甲酰-L-丝氨酸甲酯水解的动力学。通过晶格列线图分析修饰机制、底物活化催化的参数评估计算方法以及菠萝蛋白酶和木瓜蛋白酶催化水解中假定的非生产性结合的后果。
Biochem J. 1974 Aug;141(2):365-381. doi: 10.1042/bj1410365.
6
Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.使用双质子态亲电试剂作为反应性探针表征木瓜蛋白酶活性中心。在不间断的半胱氨酸-25-组氨酸-159相互作用系统中亲核反应性的证据。
Biochem J. 1978 May 1;171(2):385-401. doi: 10.1042/bj1710385.
7
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole as a reactivity probe for the investigation of the thiol proteinases. evidence that ficin and bromelain may lack carboxyl groups conformationally equivalent to that of aspartic acid-158 of papain.4-氯-7-硝基苯并-2-恶唑-1,3-二氮唑作为研究硫醇蛋白酶的反应性探针。有证据表明，无花果蛋白酶和菠萝蛋白酶可能缺乏与木瓜蛋白酶天冬氨酸-158构象相当的羧基。
Biochem J. 1976 Nov;159(2):235-44. doi: 10.1042/bj1590235.
8
PH-dependence of the steady-state rate of a two-step enzymic reaction.两步酶促反应稳态速率的pH依赖性
Biochem J. 1976 Apr 1;155(1):61-70. doi: 10.1042/bj1550061.
9
A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.一种对硫醇基团具有绝对和选择性特异性的报告基团递送系统以及一种含有7-硝基苯并-2-恶唑-1,3-二氮杂环戊二烯部分的改进型荧光探针。
Biochem J. 1975 Nov;151(2):417-32. doi: 10.1042/bj1510417.
本文引用的文献
1
PURIFICATION AND PHYSICAL CHARACTERIZATION OF STEM BROMELAIN.茎菠萝蛋白酶的纯化及物理特性研究
Biochemistry. 1964 Jan;3:48-55. doi: 10.1021/bi00889a009.
2
KINETIC STUDIES OF BROMELAIN CATALYSIS.菠萝蛋白酶催化的动力学研究。
Biochemistry. 1963 Nov-Dec;2:1439-44. doi: 10.1021/bi00906a041.
3
A novel reactivity of papain and a convenient active site titration in the presence of other thiols.木瓜蛋白酶的一种新反应活性以及在其他硫醇存在下简便的活性位点滴定法。
FEBS Lett. 1970 Jul 29;9(2):113-116. doi: 10.1016/0014-5793(70)80327-1.
4
Evidence for glycoprotein nature of stem bromelain. Isolation of a glycopeptide.菠萝蛋白酶原糖蛋白性质的证据。一种糖肽的分离。
Biochemistry. 1967 Dec;6(12):3730-6. doi: 10.1021/bi00864a015.
5
The preparation and some properties of bromelain covalently attached to O-(carboxymethyl)-cellulose.共价连接到O-（羧甲基）纤维素上的菠萝蛋白酶的制备及其某些性质
Eur J Biochem. 1968 Dec 5;6(4):565-71. doi: 10.1111/j.1432-1033.1968.tb00482.x.
6
Reactivities of the various protonic states in the reactions of papain and of L-cysteine with 2,2'- and with 4,4'- dipyridyl disulphide: evidence for nucleophilic reactivity in the un-ionized thiol group of the cysteine-25 residue of papain occasioned by its interaction with the histidine-159-asparagine-175 hydrogen-bonded system.木瓜蛋白酶和L-半胱氨酸与2,2'-二吡啶二硫化物和4,4'-二吡啶二硫化物反应中各种质子态的反应活性：木瓜蛋白酶中半胱氨酸-25残基的未电离硫醇基团因其与组氨酸-159-天冬酰胺-175氢键系统相互作用而产生亲核反应活性的证据。
Biochem J. 1972 Jun;128(2):471-4. doi: 10.1042/bj1280471.
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