葡萄糖与肽的反应。
A reaction of glucose with peptides.
作者信息
Dixon H B
出版信息
Biochem J. 1972 Aug;129(1):203-8. doi: 10.1042/bj1290203.
Abstract
Valylhistidine (Val-His) reacts with glucose (Glc) in a mixture of pyridine and acetic acid to form glucosylvalylhistidine (Glc-Val-His). The pK of the alpha-amino group is thereby lowered to about 5.6 as judged by electrophoretic mobility. The reaction: [Formula: see text] also occurs in an aqueous solution of pyridine and acetic acid of pH6.2 at 50 degrees C, in which it exhibits a half-time of about 30h and a dissociation constant of about 0.3m. Isoleucyltyrosine and glucose react similarly in aqueous solution. The Glc-Val-His has the chromatographic, electrophoretic and chemical properties reported by Holmquist & Schroeder (1966a) for the substance released by proteolysis from the N-terminus of the beta-chains of haemoglobin A(Ic); the value of the dissociation constant means that the concentration of haemoglobin A(Ic) found naturally could be explained by reaction of haemoglobin A with the blood glucose.
摘要
缬氨酰组氨酸（Val-His）在吡啶和乙酸的混合物中与葡萄糖（Glc）反应生成葡糖基缬氨酰组氨酸（Glc-Val-His）。根据电泳迁移率判断，α-氨基的pK值由此降低至约5.6。该反应：[化学式：见原文]在50℃、pH6.2的吡啶和乙酸水溶液中也会发生，在其中其半衰期约为30小时，解离常数约为0.3m。异亮氨酰酪氨酸和葡萄糖在水溶液中反应类似。Glc-Val-His具有Holmquist和Schroeder（1966a）报道的从血红蛋白A（Ic）β链N端经蛋白水解释放出的物质的色谱、电泳和化学性质；解离常数的值意味着天然存在的血红蛋白A（Ic）的浓度可以用血红蛋白A与血糖的反应来解释。
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