Weiss S J, Sagone A L
Biochim Biophys Acta. 1979 Jul 18;585(4):620-9. doi: 10.1016/0304-4165(79)90194-6.
The role of sulfhydryls in the protection of human polymorphonuclear neutrophils against extracellular oxidant attack was investigated by simultaneously exposing polymorphonuclear neutrophils to the thiol-oxidizing agent diamide and the oxidant-generating system xanthine-xanthine oxidase. Neither diamide nor the oxidants generated by the xanthine-xanthine oxidase system alone impaired the burst in chemiluminescence, hexose monophosphate shunt activity or formate oxidation normally seen during polymorphonuclear neutrophil phagocytosis. Incubation of the polymorphonuclear neutrophils simultaneously with diamide and xanthine-xanthine oxidase markedly impaired polymorphonuclear neutrophil phagocytosis, hexose monophosphate shunt activity, chemiluminescence and formate oxidation. Although the polymorphonuclear neutrophils exposed to diamide and xanthine-xanthine oxidase did not respond to a variety of phagocytizable stimuli, trypan blue exclusion was normal and hexose monophosphate shunt activity could be stimulated by diamide. The damaging effect of the diamide xanthine-xamthine oxidase system could be blocked by the addition of superoxide dismutase or catalase, but not by hydroxyl radical or singlet oxygen scavengers. We hypothesize that an unidentified population of thiols may play a role in protecting the polymorphonuclear neutrophil from endogenously derived oxidants.
通过将多形核中性粒细胞同时暴露于硫醇氧化剂二酰胺和产氧化剂系统黄嘌呤-黄嘌呤氧化酶，研究了巯基在保护人类多形核中性粒细胞免受细胞外氧化剂攻击中的作用。单独的二酰胺或黄嘌呤-黄嘌呤氧化酶系统产生的氧化剂均未损害多形核中性粒细胞吞噬作用过程中正常出现的化学发光爆发、磷酸己糖旁路活性或甲酸盐氧化。将多形核中性粒细胞与二酰胺和黄嘌呤-黄嘌呤氧化酶同时孵育，会显著损害多形核中性粒细胞的吞噬作用、磷酸己糖旁路活性、化学发光和甲酸盐氧化。尽管暴露于二酰胺和黄嘌呤-黄嘌呤氧化酶的多形核中性粒细胞对多种可吞噬刺激无反应，但台盼蓝排斥试验正常，且二酰胺可刺激磷酸己糖旁路活性。添加超氧化物歧化酶或过氧化氢酶可阻断二酰胺-黄嘌呤-黄嘌呤氧化酶系统的损伤作用，但羟基自由基或单线态氧清除剂则不能。我们推测，一种未明确的巯基群体可能在保护多形核中性粒细胞免受内源性氧化剂的侵害中发挥作用。
