Kelman L, Saunders S J, Wicht S, Frith L, Corrigall A, Kirsch R E, Terblanche J
Biochem J. 1972 Oct;129(4):805-9. doi: 10.1042/bj1290805.
Albumin synthesis was measured in the isolated perfused rat liver by using the livers of both well-fed and starved rats. Starvation markedly decreased albumin synthesis. The livers from starved rats were unable to increase synthesis rates after the addition to the perfusates of single amino acids or the addition of both glucagon and tryptophan. Arginine, asparagine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan and valine, added together to ten times their normal peripheral blood concentrations, restored synthesis rates to normal. The plasma aminogram (i.e. the relative concentrations, of amino acids) was altered by depriving rats of protein for 48h. The use of blood from the deprived rats as perfusate, instead of normal blood, decreased albumin synthesis rates significantly by livers obtained from well-fed rats. The addition of single amino acids, including the non-metabolizable amino acid, alpha-aminoisobutyric acid, to the above mixture increased albumin synthesis rates to normal values. It is concluded that amino acids play an important role in the control of albumin synthesis and that more than one mechanism is probably involved.
通过使用喂食良好和饥饿大鼠的肝脏，在离体灌注大鼠肝脏中测量白蛋白合成。饥饿显著降低白蛋白合成。饥饿大鼠的肝脏在向灌注液中添加单个氨基酸或同时添加胰高血糖素和色氨酸后，无法提高合成速率。将精氨酸、天冬酰胺、异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、脯氨酸、苏氨酸、色氨酸和缬氨酸以其正常外周血浓度的十倍添加在一起，可将合成速率恢复至正常。通过使大鼠禁食48小时来改变血浆氨基酸谱（即氨基酸的相对浓度）。使用来自禁食大鼠的血液作为灌注液，而不是正常血液，会显著降低喂食良好大鼠肝脏的白蛋白合成速率。向上述混合物中添加单个氨基酸，包括不可代谢氨基酸α-氨基异丁酸，可将白蛋白合成速率提高至正常值。结论是氨基酸在白蛋白合成的控制中起重要作用，可能涉及不止一种机制。
