外源性δ-氨基乙酰丙酸对大鼠肝脏血红素和细胞色素的影响。
The effect of exogenous -aminolaevulinate on rat liver haem and cytochromes.
作者信息
Druyan R, Kelly A
出版信息
Biochem J. 1972 Oct;129(5):1095-9. doi: 10.1042/bj1291095.
Abstract
The activity of delta-aminolaevulinate synthetase is generally regarded as rate-limiting for hepatic haem biosynthesis. It has been suggested that cytochrome synthesis may also be regulated by changes in delta-aminolaevulinate synthetase activity. This hypothesis was studied by injecting product, delta-aminolaevulinate, into adult rats over a 4-240h period. The concentrations of hepatic mitochondrial cytochromes a, b, c and c(1) were unchanged by treatment with delta-aminolaevulinate, allylisopropylacetamide or phenobarbital. In control animals, total microsomal haem content equalled the sum of cytochromes b(5) plus P-450. After delta-aminolaevulinate administration the total amount of microsomal haem, measured as the pyridine haemochromogen, exceeded these components, indicating the formation of a ;free' haem pool. Haem synthesis does not appear rate-limiting for hepatic cytochrome synthesis in the adult rat.
摘要
δ-氨基-γ-酮戊酸合成酶的活性通常被认为是肝脏血红素生物合成的限速因素。有人提出，细胞色素合成也可能受δ-氨基-γ-酮戊酸合成酶活性变化的调节。通过在4至240小时期间向成年大鼠注射产物δ-氨基-γ-酮戊酸来研究这一假说。用δ-氨基-γ-酮戊酸、烯丙基异丙基乙酰胺或苯巴比妥处理后，肝脏线粒体细胞色素a、b、c和c1的浓度没有变化。在对照动物中，微粒体血红素总量等于细胞色素b5加P-450的总和。给予δ-氨基-γ-酮戊酸后，以吡啶血红素原测量的微粒体血红素总量超过了这些成分，表明形成了一个“游离”血红素池。在成年大鼠中，血红素合成似乎不是肝脏细胞色素合成的限速因素。
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