[噬菌体φB核酸的物理化学研究]

[Physico-chemical study of the nucleic acid of bacteriophage phiB].

作者信息

Karataev G I, Bogush V G, Rebentish B A, Plotnikova T G, Naroditskaia V A

出版信息

Mol Biol (Mosk). 1978 Mar-Apr;12(2):377-84.

Abstract

A study of physico-chemical properties of the nucleic acid of phage Brevibacterium flavum was made. It was found that the phage nucleic acid is a double-stranded DNA. The content of GC pairs was determined by the temperature of DNA melting and by the chromatographic method. In both cases it was (52 +/- 2) mole %. It has been shown that endonuclease Eco RI treatment of phiB DNA forms 12 fragments. The molecular weight of phiB DNA has been determined by two independent methods: by measuring the contous length of DNA and by the sum of molecular weights of DNA restricts obtained after hydrolysis by endonuclease Eco RI and was found to be (30 +/- 1) . 10(6) dalton. Electron microscopy investigations detected rod and circular DNA in either monomeric or dimeric forms. The polarity of DNA arrangement in the phiB phage particle was determined by using the partial denaturation maps.

摘要

对黄色短杆菌噬菌体核酸的物理化学性质进行了研究。发现该噬菌体核酸是双链DNA。通过DNA解链温度和色谱法测定了GC对的含量。在这两种情况下，其含量均为（52±2）摩尔%。已表明，用内切核酸酶Eco RI处理phiB DNA可形成12个片段。通过两种独立方法测定了phiB DNA的分子量：通过测量DNA的连续长度以及通过内切核酸酶Eco RI水解后得到的DNA片段分子量之和，发现其分子量为（30±1）×10⁶道尔顿。电子显微镜研究检测到呈单体或二聚体形式的棒状和环状DNA。通过使用部分变性图谱确定了phiB噬菌体颗粒中DNA排列的极性。