谷氨酸作为大鼠小脑颗粒细胞的神经递质：电生理学证据。
Glutamate as the neurotransmitter of cerebellar granule cells in the rat: electrophysiological evidence.
作者信息
Stone T W
出版信息
Br J Pharmacol. 1979 Jun;66(2):291-6. doi: 10.1111/j.1476-5381.1979.tb13678.x.
Abstract
1 Glutamate and the excitatory aminoacid antagonist, alpha-aminoadipic acid (alphaAA), have been applied by microiontophoresis to Purkinje cells in the rat cerebellum. 2 Glutemate produced excitation of Purkinje cells and alpha AA selectively reduced that excitation without affecting responses to acetylcholine or hydrogen ions. 3 Monosynaptic spikes were evoked in Purkinje cells by stimulating the parallel fibres. alpha AA had little effect on these spikes when applied alone. 4 When the Purkinje cell excitability was reduced by the iontophoresis of gamma-aminobutyric acid, alpha AA then produced railure of the monosynaptic spike on 10 of 13 Purkinje cells, in doses shown to be selectively antagonistic towards aminoacids. 5 These results support neurochemical evidence that glutamic acid may be the neurotransmitter released by granule cell parallel fibres.
摘要
已通过微离子电泳法将谷氨酸和兴奋性氨基酸拮抗剂α - 氨基己二酸（αAA）施加于大鼠小脑的浦肯野细胞。
谷氨酸使浦肯野细胞兴奋，而αAA选择性地降低了这种兴奋，且不影响对乙酰胆碱或氢离子的反应。
通过刺激平行纤维在浦肯野细胞中诱发单突触峰电位。单独施加αAA时对这些峰电位影响很小。
当通过γ - 氨基丁酸的离子电泳降低浦肯野细胞兴奋性时，αAA在13个浦肯野细胞中的10个细胞上导致单突触峰电位消失，所用剂量显示对氨基酸具有选择性拮抗作用。
这些结果支持了神经化学证据，即谷氨酸可能是颗粒细胞平行纤维释放的神经递质。
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