Libby P R
Biochem J. 1972 Dec;130(3):663-9. doi: 10.1042/bj1300663.
The effect of oestradiol treatment on the acetylation of histones of the immature rat uterus has been studied. A 10mug dose of oestradiol causes a 70% increase at 5min and a 140% increase at 10min after administration in the labelling of the histone fraction F2+F3. No effect of oestradiol is seen on the labelling of histones F1 or acidic non-histone chromatin proteins. The oestradiol stimulation is seen in animals pretreated with either cycloheximide or actinomycin D. The stimulation of labelling caused by oestradiol is completely abolished by pretreatment of the animals with the anti-oestrogen, nafoxidine. The stimulation is given by lower doses of oestradiol, by stilboestrol and oestriol, but is not given by testosterone. These results suggest that stimulation of histone acetylation in the uterus is the earliest known effect of the hormone on its target tissue.
已对雌二醇处理对未成熟大鼠子宫组蛋白乙酰化的影响进行了研究。给予10微克剂量的雌二醇后，在5分钟时组蛋白组分F2 + F3的标记增加70%，在10分钟时增加140%。未观察到雌二醇对组蛋白F1或酸性非组蛋白染色质蛋白的标记有影响。在用环己酰亚胺或放线菌素D预处理的动物中也可见到雌二醇的刺激作用。用抗雌激素药物萘福昔定预处理动物后，雌二醇引起的标记刺激作用完全消失。较低剂量的雌二醇、己烯雌酚和雌三醇可产生刺激作用，但睾酮则无此作用。这些结果表明，子宫中组蛋白乙酰化的刺激是该激素对其靶组织已知的最早作用。
