Chatterjee A N, Young F E
J Bacteriol. 1972 Jul;111(1):220-30. doi: 10.1128/jb.111.1.220-230.1972.
Temperature-sensitive mutants of Staphylococcus aureus H which require 1.0 m NaCl for growth at 42 C can be divided into two major classes. Most of the mutants (class A) do not accumulate nucleotide precursors of cell wall biosynthesis in the absence of salt at the nonpermissive temperatures, whereas the class B mutants accumulate these precursors. The most extensively studied mutant RUS 1 (carrying peg-1) is defective in biosynthesis of peptidoglycan at the nonpermissive conditions as evidenced by: (i) reduced incorporation of cell wall precursors into peptidoglycan; (ii) accumulation of the nucleotide, uridine diphosphate (UDP) muramyl-l-alanyl-d-glutamic acid; (iii) reduced specific activity of UDP N-acetylmuramyl (MurNAc)-l-alanyl-d-glutamate: l-lysine ligase (EC 6.3.2.7); and (iv) an increased susceptibility to lysis with sodium dodecyl sulfate. Addition of 1.0 m NaCl reverses these defects with the exception of the specific activity of UDP-MurNAc-l-alanyl-d-glutamate: l-lysine ligase. Nevertheless, the structure of the cell wall is normal at the nonpermissive conditions if 1.0 m NaCl is present. An alteration in the binding of a fluorescent dye, 8-anilino-1-napthalene-4-sulfonic acid at the nonpermissive conditions in the absence of 1.0 m NaCl suggests that there may also be defects in the membrane in this strain.
金黄色葡萄球菌H的温度敏感突变体在42℃下生长需要1.0m NaCl，可分为两大类。大多数突变体（A类）在非允许温度下无盐存在时不会积累细胞壁生物合成的核苷酸前体，而B类突变体会积累这些前体。研究最广泛的突变体RUS 1（携带peg - 1）在非允许条件下肽聚糖生物合成存在缺陷，证据如下：（i）细胞壁前体掺入肽聚糖的量减少；（ii）核苷酸尿苷二磷酸（UDP）胞壁酰 - l - 丙氨酰 - d - 谷氨酸积累；（iii）UDP N - 乙酰胞壁酰（MurNAc） - l - 丙氨酰 - d - 谷氨酸：l - 赖氨酸连接酶（EC 6.3.2.7）的比活性降低；（iv）对十二烷基硫酸钠裂解的敏感性增加。添加1.0m NaCl可逆转这些缺陷，但UDP - MurNAc - l - 丙氨酰 - d - 谷氨酸：l - 赖氨酸连接酶的比活性除外。然而，如果存在1.0m NaCl，在非允许条件下细胞壁结构正常。在无1.0m NaCl的非允许条件下，荧光染料8 - 苯胺基 - 1 - 萘 - 4 - 磺酸结合的改变表明该菌株的膜可能也存在缺陷。
