Good C M, Tipper D J
J Bacteriol. 1972 Jul;111(1):231-41. doi: 10.1128/jb.111.1.231-241.1972.
Temperature-sensitive (ts) mutants of Staphylococcus aureus with defective cell wall biosynthesis have been differentiated from other ts mutants by their ability to grow at the restrictive temperature (43 C) in the presence of 1 m NaCl. Under all conditions they possess normal colonial and cellular morphology at the level of resolution of the light microscope and are, therefore, not protoplasts. However, differences between mutant and wild-type cells can be seen by scanning electron microscopy. Many of the mutants contained concentrations of nucleotide precursors of peptidoglycan synthesis in excess of those present in wild-type cells, at both 30 and 43 C. The types of peptidoglycan precursors accumulated by six of the mutants have been determined, and specific enzymatic defects in three of these have been identified.
细胞壁生物合成存在缺陷的金黄色葡萄球菌温度敏感（ts）突变体，已通过其在1 m NaCl存在下于限制温度（43℃）生长的能力，与其他ts突变体区分开来。在所有条件下，它们在光学显微镜的分辨率水平上具有正常的菌落和细胞形态，因此不是原生质体。然而，通过扫描电子显微镜可以看出突变细胞与野生型细胞之间的差异。在30℃和43℃时，许多突变体中肽聚糖合成的核苷酸前体浓度都超过了野生型细胞中的浓度。已经确定了六个突变体积累的肽聚糖前体类型，并确定了其中三个突变体的特定酶缺陷。
