Jones G
Clin Exp Immunol. 1972 Nov;12(3):403-7.
Mouse peripheral blood lymphocytes were cultured with phytohaemagglutinin or Concanavalin A and at various times antiserum to each mitogen was added. Stimulation could be completely blocked for 5–6 hr and partially inhibited for at least 48 hr, and probably longer. The block produced by antiserum was specific and dependent on the relative concentrations of mitogen and antiserum. The gradual release of activation from the requirement for mitogen was not due to the production of a soluble non-specific mediator since the stimulatory activity of culture supernatants of fresh lymphocytes could be completely blocked in turn by antiserum to the original mitogen. Other experiments made the operation of a locally concentrated mediator unlikely. These data support a direct proliferative mechanism of lymphocyte transformation to these mitogens.
将小鼠外周血淋巴细胞用植物血凝素或刀豆球蛋白A进行培养，并在不同时间加入针对每种促有丝分裂原的抗血清。刺激作用可被完全阻断5 - 6小时，并至少在48小时内受到部分抑制，可能时间更长。抗血清产生的阻断作用具有特异性，且取决于促有丝分裂原和抗血清的相对浓度。从对促有丝分裂原的需求中逐渐释放激活作用并非由于产生了一种可溶性非特异性介质，因为新鲜淋巴细胞培养上清液的刺激活性又可被针对原始促有丝分裂原的抗血清完全阻断。其他实验使得局部浓缩介质起作用的可能性不大。这些数据支持淋巴细胞向这些促有丝分裂原转化的直接增殖机制。
