通过区带离心分离培养肥大细胞在细胞周期中的核蛋白变化。

Biochem J. 1972 Aug;128(5):1213-9. doi: 10.1042/bj1281213.

Exponentially grown mouse mast cells (cell line P815, strain Y) were separated by zonal centrifugation on a Ficoll gradient. Fractions were allocated to different phases of the cell cycle according to the specific radioactivity of their DNA. 2. Histones were extracted and their thiol content was analysed. The proportion of reduced thiol increased in S phase, decreasing subsequently. 3. The phosphate content of histone F1 and of the other histones reached a peak in early and later S phase respectively. The incorporation of (32)P into these fractions showed a corresponding increase. 4. The timing of histone synthesis was examined. Incorporation of (14)C-labelled amino acids into the histone fractions took place at the same times as phosphorylation. 5. Acid nuclear proteins differ from the histones in incorporating labelled amino acids and (32)P fairly constantly through the cell cycle.

将指数生长的小鼠肥大细胞（细胞系P815，Y株）在Ficoll梯度上进行区带离心分离。根据各组分DNA的比放射性将其分配到细胞周期的不同阶段。2. 提取组蛋白并分析其巯基含量。还原型巯基的比例在S期增加，随后降低。3. 组蛋白F1和其他组蛋白的磷酸盐含量分别在S期早期和后期达到峰值。（32）P掺入这些组分中呈现相应增加。4. 检查组蛋白合成的时间。（14）C标记的氨基酸掺入组蛋白组分的时间与磷酸化时间相同。5. 酸性核蛋白与组蛋白不同，在整个细胞周期中相当稳定地掺入标记氨基酸和（32）P。

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核心技术专利：CN118964589B侵权必究

Biochem J. 1972 Aug;128(5):1213-9. doi: 10.1042/bj1281213.

Exponentially grown mouse mast cells (cell line P815, strain Y) were separated by zonal centrifugation on a Ficoll gradient. Fractions were allocated to different phases of the cell cycle according to the specific radioactivity of their DNA. 2. Histones were extracted and their thiol content was analysed. The proportion of reduced thiol increased in S phase, decreasing subsequently. 3. The phosphate content of histone F1 and of the other histones reached a peak in early and later S phase respectively. The incorporation of (32)P into these fractions showed a corresponding increase. 4. The timing of histone synthesis was examined. Incorporation of (14)C-labelled amino acids into the histone fractions took place at the same times as phosphorylation. 5. Acid nuclear proteins differ from the histones in incorporating labelled amino acids and (32)P fairly constantly through the cell cycle.

将指数生长的小鼠肥大细胞（细胞系P815，Y株）在Ficoll梯度上进行区带离心分离。根据各组分DNA的比放射性将其分配到细胞周期的不同阶段。2. 提取组蛋白并分析其巯基含量。还原型巯基的比例在S期增加，随后降低。3. 组蛋白F1和其他组蛋白的磷酸盐含量分别在S期早期和后期达到峰值。（32）P掺入这些组分中呈现相应增加。4. 检查组蛋白合成的时间。（14）C标记的氨基酸掺入组蛋白组分的时间与磷酸化时间相同。5. 酸性核蛋白与组蛋白不同，在整个细胞周期中相当稳定地掺入标记氨基酸和（32）P。