从组蛋白F1制剂中分离出的一种非组蛋白的特性鉴定。
The characterization of a non-histone protein isolated from histone F1 preparations.
作者信息
Smith J A, Stocken L A
出版信息
Biochem J. 1973 Apr;131(4):859-61. doi: 10.1042/bj1310859.
Abstract
A non-histone protein was obtained by extraction of nuclei derived from rat liver or thymus or ascites-hepatoma cells with 5% (w/v) HClO(4). Separation from histone F1 was achieved by chromatography on DEAE-cellulose. The purified component P1 was characterized and the formation of complexes with histone F1 and polylysine was studied.
摘要
通过用5%（w/v）的高氯酸提取源自大鼠肝脏、胸腺或腹水肝癌细胞的细胞核，获得了一种非组蛋白。通过在二乙氨基乙基纤维素上进行色谱分离，将其与组蛋白F1分离。对纯化的组分P1进行了表征，并研究了它与组蛋白F1和聚赖氨酸形成复合物的情况。
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