Unger-Ullmann C, Modak S P
Differentiation. 1979;12(3):135-44. doi: 10.1111/j.1432-0436.1979.tb00999.x.
Histones from 19-day-old chick embryo lens epithelium, lens fibers, liver, brain, and erythrocytes were electrophoresed in polyacrylamide gels using buffers containing sodium dodecylsulfate, acetic acid urea, or mixtures of Triton X-100 acetic acid urea. In the last two buffer systems, histone bands were characterized by their apparent molecular weights determined by electrophoresis in the second dimension in sodium dodecylsulfate containing polyacrylamide gels. From the densitograms of the stained gels, the relative proportion of protein in different histone bands was estimated. With the exception of the erythrocyte-specific histone H5, all histones from different tissues examined at any of the gel systems migrated with the same mobilities. In lens epithelium and lens fibers, all histones were present in identical proportions. As compared to liver and brain, the total amount of histone Hl was significantly lower in lens cells and erythrocytes, possibly reflecting differences between the differentiated states. However, no tissue-specific differences were found in the relative distribution of histone Hl I and Hl II among lens epithelium, lens fiber, liver and, brain, but a threefold higher Hl I : Hl II ratio (0.5--0.7) was found in erythrocytes.
采用含有十二烷基硫酸钠、醋酸尿素或 Triton X - 100 醋酸尿素混合物的缓冲液，对 19 日龄鸡胚晶状体上皮、晶状体纤维、肝脏、大脑和红细胞中的组蛋白进行聚丙烯酰胺凝胶电泳。在最后两种缓冲系统中，组蛋白条带通过在含十二烷基硫酸钠的聚丙烯酰胺凝胶中进行二维电泳所测定的表观分子量来表征。根据染色凝胶的光密度图，估算不同组蛋白条带中蛋白质的相对比例。除红细胞特异性组蛋白 H5 外，在任何凝胶系统中检测的不同组织的所有组蛋白均以相同的迁移率迁移。在晶状体上皮和晶状体纤维中，所有组蛋白的比例相同。与肝脏和大脑相比，晶状体细胞和红细胞中组蛋白 H1 的总量显著较低，这可能反映了分化状态的差异。然而，在晶状体上皮、晶状体纤维、肝脏和大脑中，组蛋白 H1I 和 H1II 的相对分布未发现组织特异性差异，但在红细胞中发现 H1I : H1II 的比例高出三倍（0.5 - 0.7）。
