急性炎症反应期间库普弗细胞的起源与动力学

Origin and kinetics of Kupffer cells during an acute inflammatory response.

作者信息

Diesselhoff-den Dulk M M, Crofton R W, van Furth R

出版信息

Immunology. 1979 May;37(1):7-14.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1457291/

Abstract

The course of the increased number of liver macrophages and the origin of these cells were studied after intravenous stimulation by zymosan, stilboestrol, or corynebacterium. The macrophages were isolated by digestion of the liver with pronase and DNAase. Zymosan doubled the number of liver macrophages per gram of liver and led to a four- to five-fold increase in the number of blood monocytes, whereas stilboestrol induced a four-fold increase of liver macrophages and a two-fold increase of blood monocytes. Corynebacterium administration gave a two-fold rise in the number of liver macrophages and a six-fold increase of blood monocytes. The labelling index of the liver macrophages showed a transient but marked increase after the administration of zymosan or stilboestrol, but returned to approximately normal values 4 days after the stimulus. Hydrocortisone given 48 h before zymosan prevented this increase in the labelling index of liver macrophages, thus demonstrating that the mononuclear phagocytes labelled had recently been recruited from the bone marrow to the liver. Stimulation by stilboestrol or zymosan of mice labelled with [H]-thymidine caused an increase in the number of labelled liver macrophages and blood monocytes as compared with the numerical course of the labelled Kupffer cells and monocytes in untreated mice. It may be concluded that this increase is attributable to the increased influx into the circulation of labelled monocytes from the bone marrow, which in turn migrate to the liver in larger numbers than are seen in the normal steady state. Local proliferation could not have been responsible for the increased number of labelled liver macrophages, because free [H]-thymidine was no longer available when the stimulus was applied. Evidence supporting the bone marrow origin of the increased number of monocytes and liver macrophages after intravenous stimulation was provided by the course of the number of monocytes and liver macrophages in hydrocortisone-treated mice given zymosan as stimulus.

摘要

研究了经静脉注射酵母聚糖、己烯雌酚或棒状杆菌刺激后，肝脏巨噬细胞数量增加的过程及其细胞来源。通过用链霉蛋白酶和脱氧核糖核酸酶消化肝脏来分离巨噬细胞。酵母聚糖使每克肝脏中的肝脏巨噬细胞数量增加一倍，并导致血液单核细胞数量增加四至五倍，而己烯雌酚使肝脏巨噬细胞数量增加四倍，血液单核细胞数量增加两倍。注射棒状杆菌使肝脏巨噬细胞数量增加两倍，血液单核细胞数量增加六倍。肝脏巨噬细胞的标记指数在注射酵母聚糖或己烯雌酚后出现短暂但显著的增加，但在刺激后4天恢复到大致正常的值。在注射酵母聚糖前48小时给予氢化可的松可阻止肝脏巨噬细胞标记指数的这种增加，从而表明标记的单核吞噬细胞最近是从骨髓招募到肝脏的。与未处理小鼠中标记的库普弗细胞和单核细胞的数量变化过程相比，用[H] - 胸腺嘧啶核苷标记的小鼠经己烯雌酚或酵母聚糖刺激后，标记的肝脏巨噬细胞和血液单核细胞数量增加。可以得出结论，这种增加归因于来自骨髓的标记单核细胞向循环中的流入增加，这些单核细胞进而以比正常稳态中更多的数量迁移到肝脏。局部增殖不可能是标记的肝脏巨噬细胞数量增加的原因，因为在施加刺激时游离的[H] - 胸腺嘧啶核苷已不再可用。氢化可的松处理的小鼠在给予酵母聚糖作为刺激后，单核细胞和肝脏巨噬细胞数量的变化过程为静脉刺激后单核细胞和肝脏巨噬细胞数量增加源于骨髓提供了证据。