变形链球菌中磷酸烯醇丙酮酸依赖性蔗糖磷酸转移酶系统的特性分析
Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans.
作者信息
St Martin E J, Wittenberger C L
出版信息
Infect Immun. 1979 Jun;24(3):865-8. doi: 10.1128/iai.24.3.865-868.1979.
Abstract
A phosphoenolpyruvate-dependent sucrose phosphotransferase system has been identified in Streptococcus mutans. Sucrose phosphotransferase activity was inducible by sucrose and had an apparent Km for sucrose of 70 microM. The product of the sucrose phosphotransferase reaction was isolated and identified as sucrose phosphate. Additional analysis revealed that the phosphate group was on the glucose moiety. Mutants unable to grow in media containing low concentrations of sucrose were isolated and found to be missing either sucrose phosphotransferase activity or the ability to hydrolyze sucrose phosphate.
摘要
在变形链球菌中已鉴定出一种磷酸烯醇丙酮酸依赖性蔗糖磷酸转移酶系统。蔗糖磷酸转移酶活性可被蔗糖诱导，其对蔗糖的表观Km值为70微摩尔。蔗糖磷酸转移酶反应的产物被分离并鉴定为蔗糖磷酸。进一步分析表明，磷酸基团在葡萄糖部分。分离出了在含有低浓度蔗糖的培养基中无法生长的突变体，发现它们要么缺乏蔗糖磷酸转移酶活性，要么缺乏水解蔗糖磷酸的能力。
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