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天狼星红和刚果红作为动物组织中淀粉样蛋白染色剂的比较。

Comparison of Sirius red and Congo red as stains for amyloid in animal tissues.

作者信息

Brigger D, Muckle R J

出版信息

J Histochem Cytochem. 1975 Jan;23(1):84-8. doi: 10.1177/23.1.46874.

DOI:10.1177/23.1.46874
PMID:46874
Abstract

Sirius red and Congo red were compared for specificity and sensitivity of amyloid staining in animal and human material. Previously described advantages of Sirius red as an amyloid dye were confirmed, as well as its disadvantage of lack of ultraviolet fluorescence. Two further disadvantages of Sirius red were discovered, both relating to animal material: (a) its unexpectedly weak staining of early experimentally induced amyloid deposits and (b) frequent uncontrollable nonspecific staining of fibrous tissues. It is therefore concluded that, overall, Congo red used by the improved alkaline technique of Puchtler, Sweat and Levine (1962) remains the best single method for demonstration of amyloid in both human and animal tissues.

摘要

对天狼星红和刚果红在动物及人体材料中淀粉样物质染色的特异性和敏感性进行了比较。天狼星红作为一种淀粉样物质染料,其先前已描述的优点得到了证实,同时也证实了它缺乏紫外线荧光这一缺点。还发现了天狼星红的另外两个缺点,均与动物材料有关:(a) 其对早期实验诱导的淀粉样沉积物染色意外地弱;(b) 纤维组织经常出现无法控制的非特异性染色。因此得出结论,总体而言,采用Puchtler、Sweat和Levine(1962年)改良碱性技术使用的刚果红,仍然是在人体和动物组织中显示淀粉样物质的最佳单一方法。

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