阴离子位点在上皮结构中的作用。阳离子聚合物对细胞膜结构的影响。
A role for anionic sites in epithelial architecture. Effects of cationic polymers on cell membrane structure.
作者信息
Quinton P M, Philpott C W
出版信息
J Cell Biol. 1973 Mar;56(3):787-96. doi: 10.1083/jcb.56.3.787.
Abstract
The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37 degrees C in Ringer's solutions containing from 0.1 to 100.0 microg/ml of cationic polymers, and subsequently was fixed with 1% OsO(4) and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures.
摘要
研究了几种阳离子聚合物（聚-L-赖氨酸、鱼精蛋白和组蛋白）对兔胆囊上皮细胞的影响，以探讨膜中负电荷位点的可能作用。将组织在含0.1至100.0微克/毫升阳离子聚合物的林格氏溶液中于37℃下孵育30分钟，随后用1%四氧化锇固定并用电子显微镜检查。所有阳离子聚合物在适当浓度下都会使膜结构产生相似的变化。相邻的膜经常融合。微绒毛等膜结构失去刚性。从渗透压损伤的细胞判断，细胞膜的通透性明显增加。这些结果表明，固定的阴离子位点在稳定上皮膜结构中起重要作用。
相似文献
1
A role for anionic sites in epithelial architecture. Effects of cationic polymers on cell membrane structure.阴离子位点在上皮结构中的作用。阳离子聚合物对细胞膜结构的影响。
J Cell Biol. 1973 Mar;56(3):787-96. doi: 10.1083/jcb.56.3.787.
2
Protamine increases the permeability of cultured epithelial monolayers.鱼精蛋白可增加培养的上皮细胞单层的通透性。
J Appl Physiol (1985). 1990 Jan;68(1):220-7. doi: 10.1152/jappl.1990.68.1.220.
3
Cationic proteins increase the permeability of cultured rabbit tracheal epithelial cells: modification by heparin and extracellular calcium.阳离子蛋白增加培养的兔气管上皮细胞的通透性：肝素和细胞外钙的修饰作用。
Exp Lung Res. 1996 Jan-Feb;22(1):85-99. doi: 10.3109/01902149609074019.
4
Protamine alters apical membrane K+ and Cl- permeability in gallbladder epithelium.鱼精蛋白可改变胆囊上皮细胞顶端膜的钾离子和氯离子通透性。
Am J Physiol. 1987 Nov;253(5 Pt 1):C662-71. doi: 10.1152/ajpcell.1987.253.5.C662.
5
Glomerular epithelium: structural alterations induced by polycations.肾小球上皮：聚阳离子诱导的结构改变。
Science. 1975 Aug 1;189(4200):390-3. doi: 10.1126/science.1145209.
6
Glomerular permeability: transfer of native ferritin in glomeruli with decreased anionic sites.肾小球通透性：阴离子位点减少的肾小球中天然铁蛋白的转运
Lab Invest. 1978 Dec;39(6):547-53.
7
Induction of interferon in L cells by polyinosinic-polycytidylic acid in the presence of cationic compounds. I. Polycations.在阳离子化合物存在的情况下，聚肌苷酸-聚胞苷酸对L细胞中干扰素的诱导作用。I. 聚阳离子
Arch Gesamte Virusforsch. 1972;36(1):71-9. doi: 10.1007/BF01250297.
8
9
Acid mucosaccharide-containing structures in the gall bladder epithelium of the rabbit as seen with the electron microscope.用电镜观察兔胆囊上皮中含酸性粘多糖的结构
Histochemistry. 1974 Jun 5;39(4):351-60. doi: 10.1007/BF00495686.
10
[Effect of histones on membrane permeability in polymorphonuclear leukocytes].[组蛋白对多形核白细胞膜通透性的影响]
Vopr Med Khim. 1973;19(6):608-13.
引用本文的文献
1
Dual gene-activated dermal scaffolds regulate angiogenesis and wound healing by mediating the coexpression of VEGF and angiopoietin-1.双基因激活的真皮支架通过介导血管内皮生长因子（VEGF）和血管生成素-1的共表达来调节血管生成和伤口愈合。
Bioeng Transl Med. 2023 Jun 25;8(5):e10562. doi: 10.1002/btm2.10562. eCollection 2023 Sep.
2
Gold Nanocages: A Novel Class of Multifunctional Nanomaterials for Theranostic Applications.金纳米笼：用于诊疗应用的新型多功能纳米材料。
Adv Funct Mater. 2010 Nov 9;20(21):3684-3694. doi: 10.1002/adfm.201001329. Epub 2010 Oct 4.
3
P2Y6 receptor-mediated proinflammatory signaling in human bronchial epithelia.P2Y6受体介导的人支气管上皮细胞促炎信号传导
PLoS One. 2014 Sep 22;9(9):e106235. doi: 10.1371/journal.pone.0106235. eCollection 2014.
4
Polymers influencing transportability profile of drug.聚合物影响药物的可传递性特征。
Saudi Pharm J. 2013 Oct;21(4):327-35. doi: 10.1016/j.jsps.2012.10.003.
5
Nanoparticles for urothelium penetration and delivery of the histone deacetylase inhibitor belinostat for treatment of bladder cancer.用于穿透尿路上皮和递送组蛋白去乙酰化酶抑制剂贝林司他的纳米颗粒，用于治疗膀胱癌。
Nanomedicine. 2013 Nov;9(8):1124-34. doi: 10.1016/j.nano.2013.05.017. Epub 2013 Jun 11.
6
The effects of size, shape, and surface functional group of gold nanostructures on their adsorption and internalization by cells.金纳米结构的尺寸、形状和表面官能团对其被细胞吸附和内化的影响。
Small. 2010 Feb 22;6(4):517-22. doi: 10.1002/smll.200901622.
7
Protective effects of taurine on protamine sulfate induced bladder damage.牛磺酸对硫酸鱼精蛋白诱导的膀胱损伤的保护作用。
World J Urol. 2006 Sep;24(4):438-44. doi: 10.1007/s00345-006-0106-y. Epub 2006 Jul 19.
8
Glycans of the trabecular meshwork in primary open angle glaucoma.原发性开角型青光眼中小梁网的聚糖
Br J Ophthalmol. 1996 May;80(5):435-44. doi: 10.1136/bjo.80.5.435.
9
Effect of protamine sulfate on the permeability properties of the mammalian urinary bladder.硫酸鱼精蛋白对哺乳动物膀胱通透性的影响。
J Membr Biol. 1993 May;133(3):227-42. doi: 10.1007/BF00232022.
10
Localisation of alpha(2,3) and alpha(2,6) linked terminal sialic acid groups in human trabecular meshwork.人小梁网中α(2,3)和α(2,6)连接的末端唾液酸基团的定位
Br J Ophthalmol. 1994 Aug;78(8):632-7. doi: 10.1136/bjo.78.8.632.
本文引用的文献
1
STUDIES ON CELL DEFORMABILITY : I. Effect of Surface Charge.细胞变形性研究：I. 表面电荷的影响。
J Cell Biol. 1965 Sep 1;26(3):735-9. doi: 10.1083/jcb.26.3.735.
2
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
3
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中，将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
4
Improvements in epoxy resin embedding methods.环氧树脂包埋方法的改进。
J Biophys Biochem Cytol. 1961 Feb;9(2):409-14. doi: 10.1083/jcb.9.2.409.
5
The ultrastructural route of fluid transport in rabbit gall bladder.兔胆囊中液体运输的超微结构途径。
J Gen Physiol. 1967 Sep;50(8):2031-60. doi: 10.1085/jgp.50.8.2031.
6
Microelectrophoretic and enzymic studies concerning the carbohydrate at the surface of rat erythrocytes.关于大鼠红细胞表面碳水化合物的微电泳和酶学研究。
Arch Biochem Biophys. 1966 Jan;113(1):77-82. doi: 10.1016/0003-9861(66)90158-5.
7
8
Glomerular sialoprotein.肾小球涎蛋白
Science. 1969 Jun 27;164(3887):1519-21. doi: 10.1126/science.164.3887.1519.
9
Cell electrophoretic mobility and cationic flux.细胞电泳迁移率和阳离子通量。
J Cell Physiol. 1969 Feb;73(1):31-6. doi: 10.1002/jcp.1040730105.
10
Ionic secretion by epithelial membranes.上皮细胞膜的离子分泌
Ciba Found Study Group. 1968;32:68-89.
Zotero 插件