Quinton P M, Philpott C W
J Cell Biol. 1973 Mar;56(3):787-96. doi: 10.1083/jcb.56.3.787.
The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37 degrees C in Ringer's solutions containing from 0.1 to 100.0 microg/ml of cationic polymers, and subsequently was fixed with 1% OsO(4) and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures.
研究了几种阳离子聚合物（聚-L-赖氨酸、鱼精蛋白和组蛋白）对兔胆囊上皮细胞的影响，以探讨膜中负电荷位点的可能作用。将组织在含0.1至100.0微克/毫升阳离子聚合物的林格氏溶液中于37℃下孵育30分钟，随后用1%四氧化锇固定并用电子显微镜检查。所有阳离子聚合物在适当浓度下都会使膜结构产生相似的变化。相邻的膜经常融合。微绒毛等膜结构失去刚性。从渗透压损伤的细胞判断，细胞膜的通透性明显增加。这些结果表明，固定的阴离子位点在稳定上皮膜结构中起重要作用。
