Yang S S, Wivel N A
J Virol. 1973 Feb;11(2):287-98. doi: 10.1128/JVI.11.2.287-298.1973.
Intracisternal A particles, known primarily for their association with various tumors, have been shown to contain high-molecular-weight (HMW) ribonucleic acid (RNA) by velocity centrifugation, using linear glycerol gradients. This HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment. By a double-labeling experiment, HMW RNA was shown to be intrinsic to intracisternal A particles and not to have resulted from cytoplasmic polysomal RNA aggregation. By a reconstitution experiment, it was determined that the results were not due to C-type virus contamination. The synthesis of HMW RNA in intracisternal A particles is inhibited by actinomycin D and ethidium bromide. These observations emphasize that there are probably some taxonomic relationships between intracisternal A particles and oncogenic RNA viruses.
脑池内A颗粒主要因与各种肿瘤相关而闻名，通过使用线性甘油梯度进行速度离心，已证明其含有高分子量（HMW）核糖核酸（RNA）。这种HMW RNA对核糖核酸酶消化和碱处理敏感，但对链霉蛋白酶处理有抗性。通过双标记实验表明，HMW RNA是脑池内A颗粒所固有的，并非由细胞质多聚核糖体RNA聚集产生。通过重组实验确定结果并非由于C型病毒污染。脑池内A颗粒中HMW RNA的合成受到放线菌素D和溴化乙锭的抑制。这些观察结果强调脑池内A颗粒与致癌RNA病毒之间可能存在一些分类学关系。
