使用放射性铁对红细胞进行电子显微镜放射自显影。
Electron microscope autoradiography of erythroid cells using radioactive iron.
作者信息
Parry D M, Blackett N M
出版信息
J Cell Biol. 1973 Apr;57(1):16-26. doi: 10.1083/jcb.57.1.16.
Abstract
The "circle analysis" method of Williams (1969) and a new improved method employing hypothetical grains described in the previous paper have been used to analyze the distribution of autoradiographic grains over erythroid bone marrow cells labeled with radioactive iron, (55)Fe. The resolution obtainable with this isotope was determined by measuring the distribution of grains about a thin line source. This distribution was also used in calculation of the circle size for the Williams's analysis and the distances of hypothetical grains for the new method. The new method provides estimates for the amount of activity in the regions of condensed and extended nuclear chromatin and for the concentration of isotope at the junction between these two areas. The possible significance of activity in this junctional region is discussed.
摘要
威廉姆斯（1969年）的“圆圈分析”方法以及前一篇论文中描述的采用假设颗粒的新改进方法，已被用于分析放射性铁（⁵⁵Fe）标记的红系骨髓细胞上放射自显影片颗粒的分布情况。通过测量围绕薄线源的颗粒分布，确定了使用该同位素可获得的分辨率。此分布还用于威廉姆斯分析的圆圈大小计算以及新方法中假设颗粒的距离计算。新方法可估算浓缩和伸展核染色质区域的活性量以及这两个区域交界处的同位素浓度。文中讨论了该交界区域活性的可能意义。
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