一种使用假设颗粒分布分析电子显微镜放射自显影片的新方法。
A new method for analyzing electron microscope autoradiographs using hypothetical grain distributions.
作者信息
Blackett N M, Parry D M
出版信息
J Cell Biol. 1973 Apr;57(1):9-15. doi: 10.1083/jcb.57.1.9.
A new method for the analysis of electron microscope autoradiographs is described which has two advantages over other methods. This method provides estimates for the activity in different regions of the section and takes into account the actual geometrical shape and position of different regions within the section. Use is made of the measured distribution of grains about a thin line source to construct a hypothetical distribution of autoradiographic grains which is then compared with the actual distribution of grains. Different activities are then ascribed to various regions until a satisfactory agreement with the autoradiographic grains is obtained using the chi(2) significance. In the next paper, an example of the use of this method is given for the distribution of grains in the nucleus of erythroid bone marrow cells labeled with radioactive iron, (55)Fe.
本文描述了一种分析电子显微镜放射自显影片的新方法，该方法相对于其他方法具有两个优点。此方法可对切片不同区域的活性进行估计，并考虑切片内不同区域的实际几何形状和位置。利用围绕细线源测量的颗粒分布来构建放射自显影颗粒的假设分布，然后将其与颗粒的实际分布进行比较。接着将不同的活性归因于各个区域，直到使用卡方显著性与放射自显影颗粒获得令人满意的一致性。在下一篇论文中，给出了使用该方法分析用放射性铁（⁵⁵Fe）标记的红系骨髓细胞核中颗粒分布的示例。
Zotero 插件