电子显微镜放射自显影术的绝对灵敏度
Absolute sensitivity of electron microscope radioautography.
作者信息
Bachmann L, Salpeter M M
出版信息
J Cell Biol. 1967 May;33(2):299-305. doi: 10.1083/jcb.33.2.299.
Abstract
A calibration method is described for measuring absolute radioautographic sensitivities under various experimental conditions. Sensitivities to (3)H and (35)S radiation, i.e. ratio of developed grains to radioactive decays in the specimen, were determined with Ilford L4 and Kodak NTE emulsions. The highest values obtained in monolayers of emulsion were (1/8) for (3)H and 1/21 for (35)S. The influence of various experimental parameters on sensitivity is described, and the possibilities for quantitative electron microscope radioautography are discussed.
摘要
本文描述了一种用于测量在各种实验条件下绝对放射自显影灵敏度的校准方法。使用伊尔福L4和柯达NTE乳剂测定了对³H和³⁵S辐射的灵敏度，即标本中显影颗粒与放射性衰变的比率。在乳剂单层中获得的最高值，³H为1/8，³⁵S为1/21。描述了各种实验参数对灵敏度的影响，并讨论了定量电子显微镜放射自显影的可能性。
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本文引用的文献
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Science. 1965 Jul 2;149(3679):60-2. doi: 10.1126/science.149.3679.60.
2
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Lab Invest. 1965 Jun;14:1041-53.
3
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J Cell Biol. 1964 Aug;22(2):469-77. doi: 10.1083/jcb.22.2.469.
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