电子显微镜放射自显影中的分辨率。III. 碘-125、重金属染色的影响以及对关键参数的重新评估。
Resolution in electron microscope autoradiography. III. Iodine-125, the effect of heavy metal staining, and a reassessment of critical parameters.
作者信息
Salpeter M M, Fertuck H C, Salpeter E E
出版信息
J Cell Biol. 1977 Jan;72(1):161-73. doi: 10.1083/jcb.72.1.161.
Abstract
Resolution for 125I-labeled specimens under electron microscope (EM) autoradiographic conditions was assessed experimentally. With this isotope the size of the silver halide crystal was the most important resolution-limiting factor. Heavy metal staining such as is routinely used in preparing animal tissues for EM autoradiography produced an improvement in resolution of approximately 15-20%. For a 500-1,000-A biological tissue section fixed with OsO4 and stained with uranyl acetate, we obtained resolution (half distance, HD) values of approximately 800 +/- 120 A using Ilford L4 emulsion and 500 +/- 70 A using a Kodak NTE-type emulsion. General aspects of resolution-limiting factors and comparison with 3H and 14C values are discussed.
摘要
在电子显微镜（EM）放射自显影条件下，对125I标记标本的分辨率进行了实验评估。对于这种同位素，卤化银晶体的大小是最重要的分辨率限制因素。在为EM放射自显影制备动物组织时常规使用的重金属染色使分辨率提高了约15 - 20%。对于用OsO4固定并用醋酸铀染色的500 - 1000埃生物组织切片，使用伊尔福L4乳剂时我们获得的分辨率（半距离，HD）值约为800±120埃，使用柯达NTE型乳剂时为500±70埃。讨论了分辨率限制因素的一般情况以及与3H和14C值的比较。
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