Asherson G L, Barnes R M
Immunology. 1973 May;24(5):885-94.
Normal mice were immunized with picryl chloride. Cr-labelled normal lymph node cells were injected 1–10 days afterwards and the net arrival of radioactivity at the draining lymph nodes estimated. The net arrival at normal unimmunized lymph nodes was 5.3 per cent of the injected dose. This rose to 13.4 per cent 1 day after immunization and then gradually declined. This increased arrival caused by picryl chloride was reduced in mice rendered unresponsive by the repeated injection of picryl sulphonic acid or given a single injection of picryl sulphonic acid 1–6 days before immunization. Blast cells labelled with IUDR from lymph nodes immunized with an unrelated agent also showed an increased arrival at draining lymph nodes. This increased arrival was reduced in unresponsive mice painted with picryl chloride. A single intravenous injection of picryl sulphonic acid increased the arrival of normal lymph node cells at lymph nodes 1 day later and this effect was also diminished in mice rendered unresponsive by repeated injections of picryl sulphonic acid. The arrival of normal lymph node cells at the spleen was reduced by painting with picryl chloride or by a single injection of picryl sulphonic acid given a few days beforehand. Painting the ears of unimmunized mice with picryl chloride increased the arrival of normal lymph node cells and blast cells at the ears. However, the arrival was unaffected by pretreatment with picryl sulphonic acid. It was concluded that the increased arrival of cells in lymph nodes caused by picryl chloride and picryl sulphonic acid had both an immunological and a chemical inflammatory component. In contrast the arrival at painted ears lacked an immunological component. This provided evidence for antibody, or antigen-sensitive cells in apparently unimmunized mice which rapidly release pharmacologically active agents on exposure to antigen.
用苦味酸对正常小鼠进行免疫。1至10天后注射铬标记的正常淋巴结细胞，并估计放射性物质在引流淋巴结处的净到达量。正常未免疫淋巴结处的净到达量为注射剂量的5.3%。免疫后1天该值升至13.4%，然后逐渐下降。苦味酸导致的这种到达量增加在通过反复注射苦味磺酸而变得无反应的小鼠中有所减少，或者在免疫前1至6天单次注射苦味磺酸的小鼠中也有所减少。用无关试剂免疫的淋巴结中用碘脱氧尿苷标记的母细胞在引流淋巴结处的到达量也增加。在用苦味酸处理的无反应小鼠中，这种增加的到达量减少。单次静脉注射苦味磺酸会使正常淋巴结细胞在1天后到达淋巴结的量增加，并且在通过反复注射苦味磺酸而变得无反应的小鼠中这种效应也减弱。通过涂抹苦味酸或提前几天单次注射苦味磺酸会减少正常淋巴结细胞到达脾脏的量。用苦味酸涂抹未免疫小鼠的耳朵会增加正常淋巴结细胞和母细胞到达耳朵的量。然而，这种到达量不受苦味磺酸预处理的影响。得出的结论是，苦味酸和苦味磺酸导致的细胞在淋巴结中到达量增加既有免疫成分又有化学炎症成分。相比之下，涂抹耳朵处的细胞到达量缺乏免疫成分。这为明显未免疫的小鼠中存在抗体或抗原敏感细胞提供了证据，这些细胞在接触抗原时会迅速释放药理活性剂。
