Zatz M M, Lance E M
J Exp Med. 1971 Jul 1;134(1):224-41. doi: 10.1084/jem.134.1.224.
The localization of syngeneic (51)Cr-labeled lymph node cells was investigated in CBA/J mice previously challenged with sheep erythrocytes, Salmonella H antigen, keyhole limpet hemocyanin, C57BL/6J skin, or rat skin. The effect of time, dose, and route of antigen administration on lymphocyte migration was studied in both primary and secondary responses. When the distribution pattern of lymphocytes was examined after 20-24 hr, it was found that increased localization of labeled cells occurred in spleen after intravenous or intraperitoneal antigen injection, and in draining lymph nodes after subcutaneous antigen injection or skin grafting. Increased localization (trapping) of lymphocytes was antigen dose dependent and could be demonstrated when 1-6 hr had elapsed between intravenous antigen administration, or when 24 hr had elapsed between subcutaneous antigen administration and intravenous cell infusion. Trapping was transient, lasting approximately 24 hr. Maximal trapping of lymphocytes in the draining nodes occurred 9 days after skin grafting in the first-set allograft response, and 3 days after grafting in the second-set allograft and first-set xenograft responses. The cell type trapped, the specificity and mechanism of action of the trap, and the role of lymphocyte trapping in the initiation of immune responses are discussed.
研究了在预先用绵羊红细胞、沙门氏菌H抗原、钥孔戚血蓝蛋白、C57BL/6J皮肤或大鼠皮肤攻击过的CBA/J小鼠中，同基因（51）铬标记的淋巴结细胞的定位情况。在初次和二次应答中，研究了抗原给药时间、剂量和途径对淋巴细胞迁移的影响。当在20 - 24小时后检查淋巴细胞的分布模式时，发现静脉内或腹腔内注射抗原后，标记细胞在脾脏中的定位增加，皮下注射抗原或皮肤移植后，在引流淋巴结中的定位增加。淋巴细胞定位（捕获）的增加是抗原剂量依赖性的，并且在静脉内给予抗原后1 - 6小时，或皮下给予抗原与静脉内输注细胞之间间隔24小时时可以得到证实。捕获是短暂的，持续约24小时。在初次同种异体移植反应中，皮肤移植后9天，在二次同种异体移植和初次异种移植反应中，移植后3天，引流淋巴结中淋巴细胞的捕获达到最大。讨论了捕获的细胞类型、捕获的特异性和作用机制以及淋巴细胞捕获在免疫应答启动中的作用。
