Talens L T, Miller M W, Miranda M
J Bacteriol. 1973 Jul;115(1):316-22. doi: 10.1128/jb.115.1.316-322.1973.
Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested the presence of a single ascospore enclosed in a thick sheath of epiplasmic materials. Two closely associated ascospores without an epiplasmic sheath were clearly distinguishable from asci of M. bicuspidata var. chathamia when similarly treated. Ultramicrotomy and TEM established conclusively that M. krissii produced a single ascospore per ascus. Neither SEM nor TEM revealed any morphological detail of the ascospores of taxonomic significance.
通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）研究了缢虫座壳孢（Metschnikowia krissii）子囊和子囊孢子的内部及表面结构，以确定其每个子囊内子囊孢子的特征和数量。用蜗牛肠道酶进行酶消化并结合SEM检查表明，存在一个被厚壁的表质物质鞘包裹的单个子囊孢子。当进行类似处理时，两个紧密相连且无表质鞘的子囊孢子与双尖虫座壳孢（M. bicuspidata var. chathamia）的子囊明显不同。超薄切片术和TEM最终确定，缢虫座壳孢每个子囊产生一个子囊孢子。SEM和TEM均未揭示子囊孢子具有分类学意义的任何形态细节。
