Giménez-Jurado G, Valderrama M J, Sá-Nogueira I, Spencer-Martins I
Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, Portugal.
Antonie Van Leeuwenhoek. 1995 Aug;68(2):101-10. doi: 10.1007/BF00873097.
The taxonomy of the yeast genus Metschnikowia has undergone profound changes over the past century. Major developments, from the capacity to obtain pure cultures of parasitic species to progress associated with the extensive use of molecular biology tools in yeast systematics, are briefly reviewed. Results from past work and new data are combined to evaluate evolutionary relationships and clarify the classification of both terrestrial and aquatic species. Recent physiological studies, including the utilization of non-conventional carbon and nitrogen sources, and characteristics like lipolytic activity and maximum temperatures for growth, are presented. The assessment of the genetic diversity within the genus by restriction analysis of the mitochondrial DNA and by the production of specific DNA probes has been explored. The results indicate the potential application of the latter in rapid identification procedures.
在过去的一个世纪里，酵母属梅奇尼科夫酵母的分类学经历了深刻的变化。本文简要回顾了一些重大进展，从获得寄生菌种纯培养物的能力，到酵母系统学中广泛使用分子生物学工具所取得的进展。结合过去的研究结果和新数据来评估进化关系，并阐明陆生和水生物种的分类。文中还介绍了近期的生理学研究，包括对非常规碳源和氮源的利用，以及脂解活性和生长最高温度等特性。通过线粒体DNA的限制性分析和特定DNA探针的制备，对该属内的遗传多样性进行了评估。结果表明，后者在快速鉴定程序中具有潜在的应用价值。
