Rice J T, Bierer B W, Dick J W
Poult Sci. 1979 Jan;58(1):18-22. doi: 10.3382/ps.0580018.
Broiler-type chickens were vaccinated orally at several ages with various dilutions of the Clemson University (CU) strain of Pasteurella multocida vaccine and then challenged orally with virulent strain P-1059 or strain X-73 of P. multocida to determine the degree of protection produced by vaccination. When vaccinated at 8 and 12 weeks of age for 2 consecutive days with a 1:133 dilution of the vaccine and then challenged at 17 and 19 weeks of age with strain X-73 and P-1059 P. multocida, respectively, no significant levels of protection were elicited in the vaccinates. Significant levels of protection were elicited in the vaccinates for 2 consecutive days at 9 weeks and at 13 weeks with 1:133 and 1:50 dilutions of the vaccine respectively, and then challenged at 16 weeks with strain X-73 and at 20 weeks with strain P-1059. Broilers vaccinated at 12 and 16 weeks for 3 consecutive days and then challenged at 20 weeks with strain X-73 showed a highly significant level of protection upon challenge when compared to the controls.
将不同稀释度的克莱姆森大学（CU）株多杀性巴氏杆菌疫苗在几个不同日龄经口接种肉用型鸡，然后经口用多杀性巴氏杆菌强毒株P - 1059或X - 73株进行攻毒，以确定疫苗接种所产生的保护程度。当在8周龄和12周龄连续两天用1:133稀释度的疫苗进行接种，然后分别在17周龄和19周龄用X - 73株和P - 1059株多杀性巴氏杆菌进行攻毒时，接种鸡未产生显著水平的保护。当分别在9周龄和13周龄连续两天用1:133和1:50稀释度的疫苗进行接种，然后在16周龄用X - 73株、在20周龄用P - 1059株进行攻毒时，接种鸡产生了显著水平的保护。在12周龄和16周龄连续三天接种疫苗，然后在20周龄用X - 73株进行攻毒的肉鸡，与对照组相比，攻毒时显示出高度显著的保护水平。
