Heath D F
Br J Exp Pathol. 1973 Aug;54(4):359-67.
The acute effects of burn injury on the transport and turnover of glucose in red cells have been studied in the rat 1·5-5 hours after injury. Post-absorptive rats received a dorsal full thickness burn to 20% of the body surface, which reduced the red cell volume from 2·46 to 2·12 ml/100 g body weight and the plasma volume from 3·72 to 2·49 ml/100 g (without affecting F) and increased the plasma glucose concentration (C) from 1·6 to 2·9 mg/ml. In controls the glucose concentration in red cells (C) was 0·48 mg/ml, independent of the value of C. The C/C ratio was about 0·3, there was a big concentration gradient across the cell membrane. After injury C rose, but from 1·5 to 5 hours after injury only at less than 1 μg/(ml red cells)/min. The C/C ratio was close to 0·3, as in controls. The slowness of the changes in C and the maintenance of a high glucose concentration gradient across the red cell membrane indicated that the transport of glucose was normal in most of the 86% of the cells remaining in the circulation. Injury increased the rate of turnover of glucose, estimated from experiments with [U-C] glucose, from 32 to 42 μg/(ml red cells)/min. This moderate increase was thought to be in the rate of glycolysis and to be a normal consequence of the increase in C after injury. It was concluded that most of the red cells remaining in the circulation after a burn injury in the rat transported and metabolized glucose normally for up to 5 hours.
在大鼠烧伤后1.5至5小时，研究了烧伤对红细胞中葡萄糖转运和周转的急性影响。禁食后的大鼠背部接受了占体表20%的全层烧伤，这使红细胞体积从2.46毫升/100克体重降至2.12毫升/100克体重，血浆体积从3.72毫升/100克降至2.49毫升/100克（不影响F），并使血浆葡萄糖浓度（C）从1.6毫克/毫升升至2.9毫克/毫升。在对照组中，红细胞中的葡萄糖浓度（C）为0.48毫克/毫升，与C的值无关。C/C比值约为0.3，细胞膜两侧存在较大的浓度梯度。烧伤后C升高，但在烧伤后1.5至5小时内仅以低于1微克/（毫升红细胞）/分钟的速度升高。C/C比值与对照组一样接近0.3。C变化的缓慢以及红细胞膜两侧高葡萄糖浓度梯度的维持表明，循环中剩余的86%的细胞中，大多数细胞的葡萄糖转运正常。根据用[U-C]葡萄糖进行的实验估计，烧伤使葡萄糖周转速率从32微克/（毫升红细胞）/分钟增加到42微克/（毫升红细胞）/分钟。这种适度的增加被认为是糖酵解速率的增加，是烧伤后C升高的正常结果。得出的结论是，大鼠烧伤后循环中剩余的大多数红细胞在长达5小时内葡萄糖转运和代谢正常。
