DiTullio N W, Stack E J
J Lipid Res. 1973 Sep;14(5):552-6.
Mice were fed a lithogenic diet consisting of Purina chow and 0.5% dehydrocholic acid (DHA group). Controls received Purina chow. Every 2 wk for 20 wk animals were killed, and biliary phospholipid, cholesterol, and bile salt concentrations were determined, as well as the extent of gallstone formation. With time there was a gradual, significant decline in the concentration and the relative composition of phospholipid in both groups compared with initial values. There was a significant increase in biliary cholesterol concentration and relative amount in the DHA group compared with the control. No significant differences were found in the relative amounts of bile salt or phospholipid between the two groups. Feeding DHA resulted in an increased concentration of bile salts and the sum of measured lipid compared with controls. After 8 wk, gallstones were found in approximately 60% of autopsied animals and correlated with increased cholesterol concentration. Our data support the hypothesis that there is a component of cholesterol secretion that may not be bile salt- or phospholipid-dependent. Our data also suggest that biliary phospholipid secretion decreases with age.
给小鼠喂食由普瑞纳饲料和0.5%脱氢胆酸组成的致石性饮食（DHA组）。对照组给予普瑞纳饲料。在20周内，每2周处死一批动物，测定胆汁中磷脂、胆固醇和胆盐的浓度以及胆结石形成的程度。随着时间的推移，与初始值相比，两组中磷脂的浓度和相对组成均逐渐显著下降。与对照组相比，DHA组胆汁中胆固醇浓度和相对含量显著增加。两组之间胆盐或磷脂的相对含量没有显著差异。与对照组相比，喂食DHA导致胆盐浓度和所测脂质总量增加。8周后，在大约60%的尸检动物中发现胆结石，且与胆固醇浓度升高相关。我们的数据支持这样的假说，即存在一部分胆固醇分泌可能不依赖于胆盐或磷脂。我们的数据还表明，胆汁中磷脂的分泌随年龄增长而减少。
