Sha'afi R I, Pascoe E
J Gen Physiol. 1973 Jun;61(6):709-26. doi: 10.1085/jgp.61.6.709.
The transport of radioactive sodium in high sodium cat red blood cells has been studied under various experimental conditions. It was found that iodoacetate (IAA) and iodoacetamide (IAM) inhibit Na influx by 50% whereas NaF has no effect. Reversible dyes, such as methylene blue (Mb), also inhibit this influx by 60%. Both IAA and Mb effects show a lag period of about 40 min. Cell starvation abolishes the volume-dependent Na influx which is generally observed in these cells. IAA reduces significantly the volume-dependent Na influx but does not inhibit it completely. 5 mM magnesium chloride produces a twofold increase in Na influx. On the other hand, MgCl(2) has no effect on Na transport in human red cells or on potassium or sulfate transport in cat red cells. The effect of MgCl(2) is quite rapid and does not interfere with the volume-dependent Na influx. This effect is abolished in starved cells. Reincubation of previously stored cells in buffered solutions containing glucose and MgCl(2) causes more than one order of magnitude increase in Na influx. These several observations are discussed in terms of the possibility of a link between Na transport and Na-Mg-activated ATPase.
在各种实验条件下，对高钠猫红细胞中放射性钠的转运进行了研究。发现碘乙酸（IAA）和碘乙酰胺（IAM）可使钠内流抑制50%，而氟化钠则无作用。诸如亚甲蓝（Mb）等可逆性染料也可使这种内流抑制60%。IAA和Mb的作用均显示约40分钟的延迟期。细胞饥饿消除了这些细胞中通常观察到的体积依赖性钠内流。IAA显著降低了体积依赖性钠内流，但并未完全抑制。5 mM氯化镁使钠内流增加两倍。另一方面，MgCl₂对人红细胞中的钠转运或猫红细胞中的钾或硫酸盐转运均无作用。MgCl₂的作用相当迅速，且不干扰体积依赖性钠内流。这种作用在饥饿细胞中消失。将先前储存的细胞在含有葡萄糖和MgCl₂的缓冲溶液中再孵育，可使钠内流增加一个多数量级。根据钠转运与钠 - 镁激活的ATP酶之间存在联系的可能性，对这些观察结果进行了讨论。
