Miles P R, Castranova V, Bleigh L, Lee P
Biochim Biophys Acta. 1977 Nov 15;471(1):105-10. doi: 10.1016/0005-2736(77)90397-2.
The effect of extracellular inorganic phosphate on Na+ movements in dog red blood cells has been studied. As the phosphate concentration is increased from 0 to 30 mM, Na+ efflux increases by 2- to 3-fold and Na+ influx increases approximately 2-fold. This enhancement of Na+ fluxes by phosphate can be prevented by the addition of iodoacetate (1 mM), an inhibitor of glycolysis, or 4-acetamido-4'-iso-thiocyantostilbene-2,2'-disulfonic acid (0.01 mM), which blocks anion transport, to the medium. The increases in Na+ movements are not caused by changes in cell volumes. These results suggest that phosphate must enter the cell to enhance Na+ fluxes and that the mechanism of action may be via a stimulatory effect on glycolysis.
研究了细胞外无机磷酸盐对犬红细胞中钠离子转运的影响。随着磷酸盐浓度从0增加到30 mM，钠离子外流增加2至3倍，钠离子内流增加约2倍。通过向培养基中添加碘乙酸盐（1 mM）（一种糖酵解抑制剂）或4-乙酰氨基-4'-异硫氰基芪-2,2'-二磺酸（0.01 mM）（一种阻断阴离子转运的物质），可以阻止磷酸盐对钠离子通量的这种增强作用。钠离子转运的增加不是由细胞体积的变化引起的。这些结果表明，磷酸盐必须进入细胞才能增强钠离子通量，其作用机制可能是通过对糖酵解的刺激作用。
