亲和柱上细胞的特异性分离。
Specific separation of cells on affinity columns.
作者信息
Truffa-Bachi P, Wofsy L
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):685-92. doi: 10.1073/pnas.66.3.685.
Abstract
Cell filtration is performed on affinity columns prepared by the chemical modification of large polyacrylamide beads. Affinity columns selectively bind cells that produce antihapten antibodies of corresponding specificity, while cells without the specific antihapten sites pass through freely and are recovered quantitatively in the eluate. The binding of the antihapten specific cells is inhibited by hapten. Such cell filtration affinity columns may be useful in specifically purifying cell populations with characteristic membrane-receptor proteins.
摘要
细胞过滤是在通过对大型聚丙烯酰胺珠进行化学修饰制备的亲和柱上进行的。亲和柱选择性地结合产生相应特异性抗半抗原抗体的细胞，而没有特定抗半抗原位点的细胞则自由通过，并在洗脱液中定量回收。半抗原可抑制抗半抗原特异性细胞的结合。这种细胞过滤亲和柱可用于特异性纯化具有特征性膜受体蛋白的细胞群体。
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Specific separation of cells on affinity columns.亲和柱上细胞的特异性分离。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):685-92. doi: 10.1073/pnas.66.3.685.
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Science. 1963 Apr 26;140(3565):405. doi: 10.1126/science.140.3565.405.
2
[Specific, complement-dependent hemolysis of sheep erythrocytes by antiserum to azo hapten groups].[偶氮半抗原基团抗血清对绵羊红细胞的特异性、补体依赖性溶血作用]
J Infect Dis. 1952 Nov-Dec;91(3):268-75. doi: 10.1093/infdis/91.3.268.
3
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J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
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Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.抗原包被柱上的细胞分离。去除高速率抗体形成细胞和免疫记忆细胞。
J Exp Med. 1969 Jan 1;129(1):23-36. doi: 10.1084/jem.129.1.23.
6
The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.交联聚丙烯酰胺微球的衍生化。为制备特殊用途的生化吸附剂而对官能团进行的可控引入。
Biochemistry. 1969 Oct;8(10):4074-82. doi: 10.1021/bi00838a026.
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