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用于测量抗体浓度和亲和力的Farr技术与平衡透析法的比较。

A comparison of the Farr technique with equilibrium dialysis for measurement of antibody concentration and affinity.

作者信息

Kim Y T, Kalver S, Siskind G W

出版信息

J Immunol Methods. 1975 Feb;6(4):347-54. doi: 10.1016/0022-1759(75)90005-8.

DOI:10.1016/0022-1759(75)90005-8
PMID:47370
Abstract

The Farr technique has been compared with equilibrium dialysis in regard to its ability to measure antibody concentration and affinity. An excellent qualitative agreement between the two techniques was obtained. Quantitatively, the Farr technique tended to underestimate antibody concentration and overestimate antibody affinity. It is likely that these observations are due to the failure of some low affinity antibodies to bind hapten under the conditions of the Farr assay.

摘要

就测量抗体浓度和亲和力的能力而言,已将法尔技术与平衡透析法进行了比较。两种技术之间获得了极好的定性一致性。在定量方面,法尔技术往往会低估抗体浓度并高估抗体亲和力。这些观察结果很可能是由于在法尔测定条件下一些低亲和力抗体未能结合半抗原所致。

相似文献

1
A comparison of the Farr technique with equilibrium dialysis for measurement of antibody concentration and affinity.用于测量抗体浓度和亲和力的Farr技术与平衡透析法的比较。
J Immunol Methods. 1975 Feb;6(4):347-54. doi: 10.1016/0022-1759(75)90005-8.
2
Technical problems in equilibrium dialysis.平衡透析中的技术问题。
Immunochemistry. 1973 Jan;10(1):3-7. doi: 10.1016/0019-2791(73)90243-7.
3
Purification of anti-dinitrophenyl antibodies and their fractionation according to affinity.抗二硝基苯基抗体的纯化及其亲和力分级分离。
J Immunol Methods. 1972 Aug;1(4):353-61. doi: 10.1016/0022-1759(72)90028-2.
4
Distribution of antibody-binding affinity. 3. Detection of low affinity antibody in the presence of high affinity antibody.抗体结合亲和力的分布。3. 在高亲和力抗体存在的情况下检测低亲和力抗体。
J Immunol. 1974 Jun;112(6):2002-12.
5
Distribution of antibody affinities: technique of measurement.抗体亲和力的分布:测量技术
Immunochemistry. 1972 Oct;9(10):987-1011. doi: 10.1016/0019-2791(72)90110-3.
6
Distribution of antibody affinities. II. Fractionation of antibody with respect to its hapten binding affinity.
Immunochemistry. 1974 Nov;11(11):685-90. doi: 10.1016/0019-2791(74)90266-3.
7
Studies on the effect of the carrier molecule on antihapten antibody synthesis. I. Effect of carrier on the nature of the antibody synthesized.载体分子对抗半抗原抗体合成影响的研究。I. 载体对合成抗体性质的影响。
J Exp Med. 1966 Apr 1;123(4):673-88. doi: 10.1084/jem.123.4.673.
8
[Regulation of antibody affinity].
Allerg Immunol (Leipz). 1973;19(2-4):238-47.
9
Carrier function in anti-hapten immune responses. I. Enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization.载体在抗半抗原免疫反应中的作用。I. 载体预免疫增强初次和二次抗半抗原抗体反应。
J Exp Med. 1970 Aug 1;132(2):261-82. doi: 10.1084/jem.132.2.261.
10
The IgM antibody site. I. Increase of binding affinity of rat 19S and 7S anti-dinitrophenyl antibodies during the course of the immune response.IgM抗体位点。I. 免疫应答过程中大鼠19S和7S抗二硝基苯抗体结合亲和力的增加。
J Immunol. 1974 Jun;112(6):2227-34.

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