Woodson R D, Wranne B, Detter J C
J Clin Invest. 1973 Nov;52(11):2717-24. doi: 10.1172/JCI107466.
Influence of altered blood oxygen affinity on maximal performance ability was evaluated in trained rats exercising to exhaustion in a graded treadmill test. Modification of blood oxygen affinity was achieved both by 2,3-diphosphoglycerate depletion, accomplished by exposure of animals to CO(2) and by exchange transfusion with blood exposed to bisulfite or stored in acid citrate dextrose, and by carbamylation of hemoglobin, produced by exchange transfusion of blood incubated with potassium cyanate. A decrease in oxygen tension at half-saturation of hemoglobin (P(50)) from 36 to 23 mm Hg produced a decrease in resting central venous oxygen pressure of about 12 mm Hg. During exercise it caused an average decrease in work performance of about 10%, which was equivalent to that performance decrement caused by a decrease in hemoglobin concentration of approximately 10%. When superimposed on anemia, this change in blood oxygen affinity again caused a similar decrease in performance over and above that due to anemia alone. A marked rightward shift of the in vivo oxygen dissociation curve during severe exercise may have compensated for the reduced in vitro P(50).
在分级跑步机测试中对训练有素的大鼠进行力竭运动，以评估血氧亲和力改变对最大运动能力的影响。通过使动物暴露于二氧化碳来消耗2,3-二磷酸甘油酸，以及用暴露于亚硫酸氢盐或储存在酸性枸橼酸盐葡萄糖溶液中的血液进行换血，来实现血氧亲和力的改变；通过用与氰酸钾孵育的血液进行换血产生血红蛋白氨甲酰化，也可实现血氧亲和力的改变。血红蛋白半饱和时的氧张力（P50）从36毫米汞柱降至23毫米汞柱，导致静息中心静脉氧压下降约12毫米汞柱。运动期间，它使工作能力平均下降约10%，这相当于血红蛋白浓度下降约10%所导致的运动能力下降。当叠加在贫血状态时，这种血氧亲和力的变化再次导致运动能力下降，且下降幅度超过仅由贫血所致的下降幅度。剧烈运动期间体内氧解离曲线明显右移，可能抵消了体外P50的降低。
