Hall B K, Tremaine R
Anat Rec. 1979 Jul;194(3):469-75. doi: 10.1002/ar.1091940312.
Whether neural crest cells from the avian embryo are determined for chondrogenesis before they begin their migration away from the neural tube (i.e., before H. H. stages 8.5--9) was investigated by establishing neural folds from embryos of H. H. stages 5--11 either in organ culture, or as grafts to the chorioallantoic membranes of host embryos. Cartilage differentiated from neural folds taken from embryos of H. H. stages 5--7 but not from those taken from older embryos. This stage specific pattern was reversed when the tissue adjacent to the neural tube was grafted to the chorioallantoic membrane. Cartilage only formed from tissues isolated later than H. H. stage 8; i.e., when these adjacent tissues contain neural crest cells. We concluded that neural crest cells are determined for chondrogenesis while still in the neural tube and before their migration to the face and head. This is in contrast to the situation in the only other group which has been examined, the urodele amphibians.
通过在器官培养中从鸡胚（H. H. 阶段5 - 11）建立神经褶，或将其移植到宿主胚胎的绒毛尿囊膜上，研究了来自鸡胚的神经嵴细胞在开始从神经管迁移之前（即H. H. 阶段8.5 - 9之前）是否已确定向软骨形成分化。从H. H. 阶段5 - 7胚胎获取的神经褶能分化出软骨，但从较老胚胎获取的神经褶则不能。当将神经管相邻组织移植到绒毛尿囊膜时，这种阶段特异性模式发生了逆转。软骨仅从H. H. 阶段8之后分离的组织形成；即当这些相邻组织包含神经嵴细胞时。我们得出结论，神经嵴细胞在仍处于神经管内且在迁移至面部和头部之前就已确定向软骨形成分化。这与唯一已研究的另一类动物——有尾两栖动物的情况相反。
