Isaacs E R, Bradley W G, Henderson G
J Neurol Neurosurg Psychiatry. 1973 Oct;36(5):813-9. doi: 10.1136/jnnp.36.5.813.
A technique of block surface-staining and serial cinematography was modified to review serial sections of normal and dystrophic muscle from the Bar Harbor 129 Re strain of mice as a preliminary study of fibre splitting in dystrophic muscle. Using this technique, muscle fibres were reconstructed for up to 1·5 mm of their length without difficulty. Split fibres were identified only when the actual separation of fibres was observed. Splitting was seen to be a significant cause of the variations in fibre diameter and was at times responsible for the formation of groups of small atrophic fibres which resembled those seen in denervation atrophy. Complex multiple splitting and recombination of daughter and parent fibres was also observed and reconstructed to scale. These results may have considerable significance for the interpretation of physiological data on both human and murine dystrophic muscle.
一种用于块面染色和连续摄影的技术被改进，用于观察来自缅因州巴尔港129系小鼠的正常和营养不良肌肉的连续切片，作为对营养不良肌肉中纤维分裂的初步研究。使用该技术，肌肉纤维可轻松重建长达1.5毫米的长度。仅当观察到纤维实际分离时才识别出分裂纤维。分裂被认为是纤维直径变化的一个重要原因，有时还导致形成类似于去神经萎缩中所见的小萎缩纤维群。还观察到子纤维和母纤维的复杂多重分裂和重组，并按比例重建。这些结果对于解释人类和小鼠营养不良肌肉的生理数据可能具有相当重要的意义。
