7-α-甲氧基头孢菌素的生物合成。分子氧的掺入。
Biosynthesis of a 7-alpha-methoxycephalosporin. Incorporation of molecular oxygen.
作者信息
O'Sullivan J, Aplin R T, Stevens C M, Abraham E P
出版信息
Biochem J. 1979 Apr 1;179(1):47-52. doi: 10.1042/bj1790047.
Abstract
A 7-alpha-methoxycephalosporin containing a carbamoyloxymethyl substituent at C-3 (cephamycin C) has been isolated from the extracellular fluid of an aqueous suspension of Streptomyces clavuligerus shaken in the presence of 18O2. The cephalosporin has been converted into its N-acetyl dimethyl ester and the distribution of 18O in the latter determined by chemical-ionization mass spectrometry. The results indicate that the oxygen atom of the methoxy group, as well as that linked to the exocyclic methylene group at C-3, is derived from molecular O2.
摘要
从在(^{18}O_2)存在下振荡的棒状链霉菌水悬浮液的细胞外液中分离出一种在C-3位含有氨甲酰氧基甲基取代基的7-α-甲氧基头孢菌素（头霉素C）。该头孢菌素已转化为其N-乙酰二甲基酯，并通过化学电离质谱法测定了后者中(^{18}O)的分布。结果表明，甲氧基的氧原子以及与C-3位环外亚甲基相连的氧原子均来源于分子氧(O_2)。
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