O'Sullivan J, Abraham E P
Biochem J. 1980 Feb 15;186(2):613-6. doi: 10.1042/bj1860613.
In the presence of S-adenosylmethionine, 2-oxoglutarate, Fe2+ and a reducing agent, cell-free extracts of Streptomyces clavuligerus convert cephalosporin C and O-carbamoyldeacetylcephalosporin C into 7 alpha-methoxy derivatives. No synthesis of a 7 alpha-methoxy derivative of deacetylcephalosporin C was detected in the system used, and the 7 alpha-methoxy derivative of deacetoxycephalosporin C was produced only in relatively small amounts. It appears that the 7 alpha-methoxy group is introduced after the cephalosporin ring system has been formed and that its introduction may represent the final step in a biosynthetic pathway.
在存在S-腺苷甲硫氨酸、2-氧代戊二酸、Fe2+和一种还原剂的情况下,棒状链霉菌的无细胞提取物可将头孢菌素C和O-氨甲酰脱乙酰头孢菌素C转化为7α-甲氧基衍生物。在所使用的体系中未检测到脱乙酰头孢菌素C的7α-甲氧基衍生物的合成,且去乙酰氧基头孢菌素C的7α-甲氧基衍生物仅以相对少量产生。似乎7α-甲氧基是在头孢菌素环系统形成后引入的,并且其引入可能代表生物合成途径中的最后一步。